Reflections on the development and application of FISH whole chromosome painting

Mutation Research. Reviews in Mutation Research
James D Tucker

Abstract

This review describes my personal reflections on the development of whole chromosome painting using fluorescence in situ hybridization and how my laboratory applied the technology in humans and in animal models. The trials and triumphs of the early years are emphasized, along with some of the scientific surprises that were encountered along the way. Scientific issues that my laboratory addressed using chromosome painting technologies are summarized and related to questions in radiation dosimetry, chemical clastogenesis, translocation persistence, and translocation frequencies in unexposed people. A description is provided of scientific controversies that were encountered and how they were resolved. I hope this paper will encourage young scientists to follow their passions and pursue their scientific dreams even if the task seems daunting and the circumstances appear exceedingly difficult. In my case the journey has been challenging, exciting, and richly rewarding on many levels.

References

Oct 1, 1975·Mutation Research·A V CarranoD Piluso
Jan 1, 1986·Teratogenesis, Carcinogenesis, and Mutagenesis·J D TuckerT M Ong
Jul 1, 1989·International Journal of Radiation Biology·J N LucasJ W Gray
Sep 1, 1989·Mutation Research·J D TuckerJ S Felton
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D PinkelJ Gray
Feb 1, 1985·Mutation Research·M Fenech, A A Morley
Jan 1, 1974·Cytogenetics and Cell Genetics·D A MillerO J Miller
Jun 12, 1970·Science·M L Pardue, J G Gall
Jan 1, 1984·Carcinogenesis·J S FeltonF T Hatch
Feb 1, 1994·Mutation Research·J R Savage, P J Simpson
Jan 1, 1995·Cytogenetics and Cell Genetics·J W BrenemanJ D Tucker
Jan 1, 1995·Cytogenetics and Cell Genetics·J D TuckerC Shasserre
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J N Lucas, R K Sachs
Apr 1, 1996·Nature Genetics·M R SpeicherD C Ward
Jul 26, 1996·Science·E SchröckT Ried
Aug 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J W BrenemanJ D Tucker
Nov 1, 1996·Mutation Research·J R Savage, J D Tucker
Jan 1, 1997·Environmental and Molecular Mutagenesis·J D TuckerD H Moore
Oct 17, 1998·Environmental and Molecular Mutagenesis·A E DirectorJ Nath
Mar 11, 1999·International Journal of Radiation Biology·K L JohnsonC R Geard
Mar 20, 1999·Mutation Research·J D TuckerJ Nath
Apr 23, 2002·Radiation Research·Shea N Gardner, James D Tucker

❮ Previous
Next ❯

Citations

Oct 5, 2019·PloS One·Diona PodushkinaEdward M Golenberg
Aug 4, 2020·Journal of Biochemistry·Lorena ZanninoMarco Biggiogera
Dec 20, 2020·Mutation Research. Reviews in Mutation Research·Richard J Albertini, Debra A Kaden

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.