Refolding of thermally and urea-denatured ribonuclease A monitored by time-resolved FTIR spectroscopy

Biochemistry
D ReinstädlerDieter Naumann

Abstract

We undertook a first detailed comparative analysis of the refolding kinetics of ribonuclease A (RNase A) by time-resolved Fourier transform infrared spectroscopy. The refolding process was initiated either by applying a temperature jump on the thermally denatured protein or by rapid dilution of a concentrated [13C]urea solution containing the chemically unfolded protein. The dead time of the injecting and mixing devices and the time-resolution of the spectrometer permitted us to monitor the refolding kinetics in a time range of 100 ms to minutes. The infrared amide I' band at 1631 cm-1 was used to directly probe the formation of beta-sheet structure during the refolding process. The aromatic ring stretching vibration of tyrosine at 1515 cm-1 was employed as a local monitor that detects changes in the tertiary structure along the folding pathway. The comparative analysis of the kinetics of the beta-sheet formation of chemically and thermally denatured ribonuclease A revealed similar folding rates and amplitudes when followed under identical refolding conditions. Therefore, our kinetic infrared studies provide evidence for a high structural similarity of urea-denatured and heat-denatured RNase A, corroborating the conclusions der...Continue Reading

References

May 1, 1991·Journal of Biomolecular NMR·K AkasakaH Nakatani
Jan 17, 1986·Biochimica Et Biophysica Acta·J M OlingerR S Brody
Jul 5, 1974·Journal of Molecular Biology·P L Privalov, N N Khechinashvili
Jan 1, 1995·Critical Reviews in Biochemistry and Molecular Biology·M Jackson, H H Mantsch
Mar 15, 1995·The Biochemical Journal·A J WhiteC W Wharton
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D FischerD Eisenberg
Jan 1, 1996·Folding & Design·R L Baldwin

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Citations

May 14, 2009·Journal of Biomolecular NMR·Iain J DayP J Hore
Apr 30, 2003·International Journal of Biological Macromolecules·Hélène GaussierMuriel Subirade
May 16, 2002·Biochimica Et Biophysica Acta·E PautheF Goubard
Aug 1, 1997·Current Opinion in Biotechnology·A R Clarke, J P Waltho
Sep 23, 2003·Biochimica Et Biophysica Acta·Christian ZscherpWerner Mäntele
Sep 14, 2013·Analytical Chemistry·Kazuhiko YamasakiTakamitsu Kohzuma
Oct 4, 2007·Journal of the American Chemical Society·Gurusamy BalakrishnanThomas G Spiro
Dec 9, 2010·Journal of the American Chemical Society·Gai LiuNoel D Lazo
Oct 24, 2002·European Journal of Biochemistry·Yong-Bin YanHai-Meng Zhou
Apr 24, 2001·Protein Science : a Publication of the Protein Society·S D SteleaT A Keiderling
Feb 6, 1999·Annual Review of Physical Chemistry·R H CallenderW H Woodruff
Feb 8, 2014·PloS One·Besnik Krasniqi, Jeremy S Lee
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·E KauffmannK Gerwert
Apr 15, 2016·Biomolecular Detection and Quantification·Oskar KarlssonMats Borén
Nov 24, 2011·Protein Science : a Publication of the Protein Society·Arnaldo L SerranoFeng Gai
Nov 14, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Alesia A TietzeAnnegret Stark
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Jun 18, 1998·Folding & Design·A NöppertG Damaschun
Jun 2, 2010·Biophysical Journal·Jean-Baptiste RougetCatherine A Royer
May 20, 2005·Applied Spectroscopy·Víctor A Lórenz-Fonfría, Esteve Padrós
Dec 25, 2000·The Journal of Biological Chemistry·P LaschT Grune
Oct 28, 2019·Journal of Biophotonics·Giuseppe BellisolaGianfelice Cinque
Dec 20, 2008·The Journal of Physical Chemistry. B·Bogusława Czarnik-MatusewiczYoung Mee Jung
Sep 4, 2010·The Journal of Physical Chemistry. B·Ryan D PensackJohn B Asbury
Sep 3, 2021·Biophysical Journal·Maria PachettiClaudio Masciovecchio
Oct 6, 2006·The Journal of Physical Chemistry. B·Gurusamy BalakrishnanThomas G Spiro
Feb 19, 2002·Chemical Reviews·Ronald T. Raines

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