Refolding studies using pressure: the folding landscape of lysozyme in the pressure-temperature plane

Biochimica Et Biophysica Acta
L SmellerKarel Heremans

Abstract

Refolding of hen egg white lysozyme after pressure unfolding was measured by FTIR spectroscopy. The high-pressure treatment was found to be useful for unfolding/refolding studies because pressure acts against aggregation, and therefore no irreversible aggregation takes place during the pressure treatment. After the release of the pressure, folding intermediate structures were found which were formed during the decompression of the lysozyme. These were aggregation prone when heated, as indicated by their lower stability against aggregation. The intermediates were only formed if the protein was unfolded, subdenaturing pressures could not populate these intermediates. We introduced the notion of a superfunnel to describe the free energy landscape of interacting polypeptide chains. This can explain the propensity of folding intermediates to aggregate. A possible Gibbs-free energy landscape for lysozyme was constructed for the whole pressure-temperature plane.

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Citations

Oct 16, 2010·European Biophysics Journal : EBJ·Judit SomkutiLászló Smeller
Jan 8, 2013·European Biophysics Journal : EBJ·Vytautas PetrauskasDaumantas Matulis
Mar 31, 2011·Journal of the American Chemical Society·Jean-Baptiste RougetCatherine A Royer
Jul 16, 2013·Biophysical Chemistry·Judit Somkuti, László Smeller
Feb 18, 2016·The Journal of Physical Chemistry. B·Maria KarayianniPetar Petrov
Jun 2, 2010·Biophysical Journal·Jean-Baptiste RougetCatherine A Royer
Sep 6, 2012·The Journal of Physical Chemistry. B·Takahiro TakekiyoYukihiro Yoshimura
Apr 20, 2012·The Journal of Physical Chemistry. B·Payel Das, Silvina Matysiak
Aug 10, 2013·The Journal of Physical Chemistry. B·Takahiro TakekiyoYukihiro Yoshimura
Mar 18, 2011·Biomacromolecules·Maria KarayianniGeorge Nounesis
Oct 25, 2011·The Journal of Physical Chemistry. B·Francesco MallamaceSow-Hsin Chen
Mar 26, 2013·Protein Engineering, Design & Selection : PEDS·Kenji OguraFuyuhiko Inagaki
Oct 6, 2010·Chemical Communications : Chem Comm·James A CracknellFraser A Armstrong
Mar 24, 2020·Biopolymers·Takahiro TakekiyoYukihiro Yoshimura
Sep 8, 2011·Physical Chemistry Chemical Physics : PCCP·Irena Danielewicz-FerchminA Ryszard Ferchmin

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