Regenerated rat fast muscle transplanted to the slow muscle bed and innervated by the slow nerve, exhibits an identical myosin heavy chain repertoire to that of the slow muscle

Histochemistry and Cell Biology
E Snoj-CvetkoIda Erzen

Abstract

The hypothesis that the limited adaptive range observed in fast rat muscles in regard to expression of the slow myosin is due to intrinsic properties of their myogenic stem cells was tested by examining myosin heavy chain (MHC) expression in regenerated rat extensor digitorum longus (EDL) and soleus (SOL) muscles. The muscles were injured by bupivacaine, transplanted to the SOL muscle bed and innervated by the SOL nerve. Three months later, muscle fibre types were determined. MHC expression in muscle fibres was demonstrated immunohistochemically and analysed by SDS-glycerol gel electrophoresis. Regenerated EDL transplants became very similar to the control SOL muscles and indistinguishable from the SOL transplants. Slow type 1 fibres predominated and the slow MHC-1 isoform was present in more than 90% of all muscle fibres. It contributed more than 80% of total MHC content in the EDL transplants. About 7% of fibres exhibited MHC-2a and about 7% of fibres coexpressed MHC-1 and MHC-2a. MHC-2x/d contributed about 5-10% of the whole MHCs in regenerated EDL and SOL transplants. The restricted adaptive range of adult rat EDL muscle in regard to the synthesis of MHC-1 is not rooted in muscle progenitor cells; it is probably due to an i...Continue Reading

References

Jan 1, 1975·Pflügers Archiv : European journal of physiology·E Gutmann, B M Carlson
Sep 1, 1979·European Journal of Biochemistry·A d'AlbisJ J Bechet
Oct 1, 1978·Experimental Eye Research·A H Neufeld
Jan 1, 1992·Reviews of Physiology, Biochemistry and Pharmacology·D Pette, G Vrbová
Jan 1, 1991·Neuromuscular Disorders : NMD·J B Miller
Dec 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P Gunning, E Hardeman
Apr 1, 1990·Developmental Biology·K CondonW J Thompson
Dec 1, 1988·The Journal of Cell Biology·E VivarelliG Cossu
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J B Miller, F E Stockdale
Mar 1, 1987·The Journal of Cell Biology·M NarusawaA M Kelly
Jun 1, 1987·Journal of Applied Physiology·C M Donovan, J A Faulkner
Dec 1, 1986·Experimental Neurology·U CarraroL Marchioro
Mar 14, 1985·Nature·R Hennig, T Lømo
Jun 1, 1973·The American Journal of Anatomy·B M Carlson
Jan 1, 1983·Medicine and Science in Sports and Exercise·B M Carlson, J A Faulkner
Apr 1, 1995·Journal of Muscle Research and Cell Motility·S E AlwayW J Roman
Nov 1, 1993·Journal of Applied Physiology·R J Talmadge, R R Roy
Aug 1, 1996·Journal of Muscle Research and Cell Motility·E Snoj-CvetkoI Erzen
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·A MAURO
May 1, 1955·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H A PADYKULA, E HERMAN

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Citations

Apr 15, 2000·The Journal of Experimental Zoology·K NakadaT Hirabayashi
Jul 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Karl J A McCullaghStefano Schiaffino
Jun 5, 2012·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·E CvetkoI Eržen
Dec 20, 2005·Journal of Muscle Research and Cell Motility·Philippe NoirezArnaud Ferry
Jun 26, 1999·Acta Physiologica Scandinavica·A FerryM Rieu
Jun 19, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Erika CvetkoIda Eržen
Jan 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Bruce M Carlson

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