Regenerating articular tissue by converging technologies.

PloS One
Lorenzo MoroniClemens A van Blitterswijk

Abstract

Scaffolds for osteochondral tissue engineering should provide mechanical stability, while offering specific signals for chondral and bone regeneration with a completely interconnected porous network for cell migration, attachment, and proliferation. Composites of polymers and ceramics are often considered to satisfy these requirements. As such methods largely rely on interfacial bonding between the ceramic and polymer phase, they may often compromise the use of the interface as an instrument to direct cell fate. Alternatively, here, we have designed hybrid 3D scaffolds using a novel concept based on biomaterial assembly, thereby omitting the drawbacks of interfacial bonding. Rapid prototyped ceramic particles were integrated into the pores of polymeric 3D fiber-deposited (3DF) matrices and infused with demineralized bone matrix (DBM) to obtain constructs that display the mechanical robustness of ceramics and the flexibility of polymers, mimicking bone tissue properties. Ostechondral scaffolds were then fabricated by directly depositing a 3DF structure optimized for cartilage regeneration adjacent to the bone scaffold. Stem cell seeded scaffolds regenerated both cartilage and bone in vivo.

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Citations

Aug 21, 2012·Acta Biomaterialia·Cédryck Vaquette, Justin Cooper-White
Oct 1, 2010·Advanced Materials·Scott J Hollister
May 28, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Stefano BenazziOttmar Kullmer
Aug 18, 2009·Journal of Tissue Engineering and Regenerative Medicine·Chiming YangHelen M Burt
May 15, 2013·Journal of Tissue Engineering and Regenerative Medicine·Lorenzo MoroniPamela Habibovic
Jul 3, 2015·Acta Biomaterialia·S M GiannitelliA Rainer
Mar 20, 2013·Biomatter·Anandkumar NandakumarPamela Habibovic
Dec 21, 2016·International Journal of Biological Macromolecules·K BalagangadharanN Selvamurugan

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Methods Mentioned

BETA
X-ray
infrared spectroscopy
scanning electron microscopy
bone marrow aspiration
scanning
x-ray diffraction
dissection

Software Mentioned

Rhinoceros®
Bioplotter

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