Regenerating Corticospinal Axons Innervate Phenotypically Appropriate Neurons within Neural Stem Cell Grafts

Cell Reports
Hiromi KumamaruMark H Tuszynski

Abstract

Neural progenitor cell grafts form new relays across sites of spinal cord injury (SCI). Using a panel of neuronal markers, we demonstrate that spinal neural progenitor grafts to sites of rodent SCI adopt diverse spinal motor and sensory interneuronal fates, representing most neuronal subtypes of the intact spinal cord, and spontaneously segregate into domains of distinct cell clusters. Host corticospinal motor axons regenerating into neural progenitor grafts innervate appropriate pre-motor interneurons, based on trans-synaptic tracing with herpes simplex virus. A human spinal neural progenitor cell graft to a non-human primate also received topographically appropriate corticospinal axon regeneration. Thus, grafted spinal neural progenitor cells give rise to a variety of neuronal progeny that are typical of the normal spinal cord; remarkably, regenerating injured adult corticospinal motor axons spontaneously locate appropriate motor domains in the heterogeneous, developing graft environment, without a need for additional exogenous guidance.

Citations

Dec 24, 2019·Frontiers in Cellular Neuroscience·Fabio LaredoAndrea Tedeschi
Nov 16, 2019·Nature Reviews. Neurology·Thomas H Hutson, Simone Di Giovanni
Sep 3, 2019·Frontiers in Cellular Neuroscience·Matthew TrawczynskiRichard G Fessler
Dec 15, 2020·Molecular Autism·Arquimedes ChefferOliver Brüstle
Mar 6, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Narjes ShahsavaniSoheila Karimi-Abdolrezaee
Apr 9, 2021·Expert Opinion on Biological Therapy·William Brett McIntyreMichael G Fehlings
Jul 21, 2021·Investigative Ophthalmology & Visual Science·Jiun L DoDerek S Welsbie
Jul 27, 2021·Journal of Neural Engineering·Laura Rodríguez DobladoManuel Monleón Pradas

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BETA
confocal microscopy

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ImageJ

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