PMID: 8586998Oct 1, 1995Paper

Regeneration in the optic nerve of adult rats: influences of cultured astrocytes and optic nerve grafts of different ontogenetic stages

Journal of Neurocytology
J SieversR Lucius

Abstract

We have studied the effects of transplanted optic nerves of different ontogenetic stages (E19 to adult), and cultured astrocytes from P2 cerebral cortex on the regeneration of axons in the optic nerve of adult rats. Regeneration was visualized by anterograde tracing with rhodamine-iso-thiocyanate. Grafts were identified with Nuclear Yellow. Astroglia within both the cut optic nerve and the transplants were detected by anti-glial fibrillary acidic protein staining. In control animals (cut optic nerve, 2-3 mm behind the optic disc), only a few neurites were found 15 days after the operation which grew randomly for short distances into the surrounding meningeal sheaths. Perinatal (E19 to P2) optic nerves induced a massive outgrowth of RITC-filled axons from the host optic nerve. The regenerating fibres grew for up to 3 mm towards the graft, ahead of glial fibrillary acidic protein-positive astroglia emanating from the host optic nerve that seemed to follow them. Although the regenerating fibres reached the grafts, they did not penetrate them. Optic nerve grafts of increasing age elicited smaller growth responses; e.g. grafts from P8 promoted only a very limited (several 100 microns) growth response, grafts from P12 and later induc...Continue Reading

References

Sep 1, 1978·Developmental Biology·P C Letourneau
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H S KeirsteadJ D Steeves
Sep 1, 1990·Journal of Neurobiology·I ShimizuA Shneiderman
Jul 1, 1989·The Journal of Cell Biology·D B Rifkin, D Moscatelli
Mar 15, 1989·The Journal of Comparative Neurology·J SieversM Berry
Oct 1, 1988·Journal of Neurocytology·K F Jackson, I D Duncan
Oct 7, 1985·Brain Research·M P DentingerC K Csiza
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Mar 1, 1983·The Journal of Cell Biology·P LiesiA Vaheri
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H W MüllerW Seifert
Jan 1, 1984·Journal of Neuroscience Research·P LiesiA Vaheri
Jun 10, 1982·Brain Research·P McConnell, M Berry
Apr 1, 1995·Journal of Neurochemistry·R LuciusR Mentlein
Oct 1, 1994·Current Opinion in Neurobiology·T N Jelsma, A J Aguayo
Jul 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R LuciusJ Sievers

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Citations

May 14, 2003·Progress in Retinal and Eye Research·Stefan IsenmannAlessandro Cellerino
Jun 9, 2000·Annual Review of Neuroscience·J L Goldberg, B A Barres
Jul 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R LuciusJ Sievers
Sep 12, 2006·Progress in Retinal and Eye Research·Alan R HarveyQi Cui
Jan 1, 2010·Cell Medicine·Hongyun HuangPaul Sanberg
Oct 23, 2002·Journal of Neuroscience Research·Bruce Teter, J Wesson Ashford
Aug 19, 2007·Journal of Glaucoma·Natalie D Bull, Keith R Martin
Nov 26, 2010·Journal of Neuro-ophthalmology : the Official Journal of the North American Neuro-Ophthalmology Society·Darcie L Moore, Jeffrey L Goldberg
Sep 1, 1999·Microscopy Research and Technique·R E BlancoC Del Cueto
Mar 6, 2003·The Anatomical Record. Part B, the New Anatomist·J G BoydR Doucette

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