Regeneration of elastic fibers by three-dimensional culture on a collagen scaffold and the addition of latent TGF-β binding protein 4 to improve elastic matrix deposition

Biomaterials
Rino AyaShigehiko Suzuki

Abstract

The objective of this study was to investigate the effects of latent TGF-β binding protein 4 (LTBP-4) on elastic fiber regeneration in three-dimensional cultures of human dermal fibroblasts (HDFs). Appropriate collagen scaffold for elastic fiber regeneration was also examined. Collagen sponges cross-linked at 120 °C and composed of small pores (25 μm on average) was favorable for elastic fiber regeneration by HDFs. Addition of LTBP-4, followed by culture for 21 days, accelerated elastic fiber accumulation within the scaffolds. Conditioned scaffolds containing either HDFs or LTBP-4-built mature elastic fibers were implanted between the dermis and the cutaneous muscle of mice. The combined use of HDFs and LTBP-4 resulted in thicker tissues containing elastic fibers. These results indicate that weakly cross-linked collagen sponges can be used as scaffolds for regenerating elastic fibers both in vitro and in vivo, and that the addition of LTBP-4 accelerates the deposition of both elastin and fibrillin-1, and increases cell proliferation. These techniques may be useful for generating cutaneous or cardiovascular tissue equivalents; furthermore, they may serve as a useful method for the three-dimensional analyses of drugs used to trea...Continue Reading

References

Mar 1, 1976·Plastic and Reconstructive Surgery·K S BhangooJ R Connelly
Jul 1, 1990·Biomaterials·S SuzukiY Ikada
Oct 1, 1993·Biomaterials·K MatsudaY Ikada
Jan 29, 2000·The Journal of Investigative Dermatology·F Duplan-PerratF Braye
Apr 27, 2001·Mechanisms of Ageing and Development·A J Bailey
Jan 24, 2002·Nature·Tomoyuki NakamuraKenneth R Chien
May 14, 2003·Trends in Cell Biology·Frederick Grinnell
Aug 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Jennifer L Long, Robert T Tranquillo
Apr 17, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Thaís P AmadeuAndréa M A Costa
Dec 5, 2006·Skin Pharmacology and Physiology·K R MewesR Graf
Mar 21, 2007·The Journal of Cell Biology·Maretoshi HiraiTomoyuki Nakamura
Nov 20, 2008·Journal of Cellular Physiology·Branka DabovicDaniel B Rifkin
Dec 4, 2008·Journal of Tissue Engineering and Regenerative Medicine·E HadjipanayiR A Brown
Jul 10, 2009·Age·Michael J Sherratt
Sep 24, 2009·Current Opinion in Cell Biology·Martin J Humphries, Albert Reynolds
Nov 3, 2009·Biochemical and Biophysical Research Communications·Mika IkedaShigehiko Suzuki
Nov 11, 2009·Cell and Tissue Research·L Bruckner-TudermanK Unsicker
Feb 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kazuo NodaTomoyuki Nakamura
Jun 20, 2014·Journal of Tissue Engineering and Regenerative Medicine·Mervyn J MerrileesThomas N Wight

❮ Previous
Next ❯

Citations

Oct 12, 2018·British Journal of Pharmacology·Yunxia WangRong Qi
Aug 21, 2018·Advanced Materials·Anna SorushanovaDimitrios I Zeugolis
Jun 20, 2019·Frontiers in Cardiovascular Medicine·Aneesh K RamaswamyJustin S Weinbaum
Feb 20, 2021·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Rodrigo Rodrigues RodriguesPedro Paulo de Andrade Santos
May 13, 2019·ACS Biomaterials Science & Engineering·Nesa FaniMohamadreza Baghaban Eslaminejad

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.