Regeneration of entorhinal fibers in mouse slice cultures is age dependent and can be stimulated by NT-4, GDNF, and modulators of G-proteins and protein kinase C

Experimental Neurology
P PrangJ P Kapfhammer

Abstract

Axonal regeneration after lesions is normally not possible in the mature central nervous system, but occurs in the embryonic and neonatal nervous system. Slice cultures offer a convenient experimental system to study the decline of axonal regeneration with increasing maturation of central nervous system tissue. We have used mouse entorhinohippocampal slice cultures to assess regeneration of entorhinal fibers after mechanical lesions in vitro. We found that entorhinal axons regenerate well in cultures derived from postnatal days 5-7 mouse pups when the lesion is made at the second and fourth days in vitro (DIV 2 and DIV 4). Only little regenerative outgrowth is seen after lesions made at DIV 6 and DIV 10. This indicates that a maturation of the cultures occurs within a short time period in vitro resulting in a loss of the regenerative potential. We have used this system to screen for neurotrophic factors and pharmacological compounds that may promote axonal regeneration. Treatments were added to the cultures 1 day before the lesion was made. We found that most added factors did not promote regeneration. Only treatment with the neurotrophic factors NT-4 and GDNF stimulated regeneration in cultures where normally little regenerati...Continue Reading

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Citations

Jul 9, 2002·The European Journal of Neuroscience·José A del RíoEduardo Soriano
Jan 25, 2005·Journal of Neurochemistry·Claudia KarlLudwig Aigner
Dec 28, 2011·Journal of Neuroinflammation·Francesco BoatoSven Hendrix
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Nov 19, 2003·The European Journal of Neuroscience·Oliver von Bohlen und HalbachKlaus Unsicker
Jun 12, 2004·Experimental Neurology·Vesna Radojevic, Josef P Kapfhammer
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Feb 3, 2004·The Journal of Comparative Neurology·Aziz HafidiDan H Sanes
Jun 3, 2008·The European Journal of Neuroscience·Brenda Bonnici, Josef P Kapfhammer
Jan 13, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ana MingoranceJosé Antonio del Río
Dec 3, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Markus HöltjeGudrun Ahnert-Hilger
Jun 2, 2021·Scientific Reports·Yuni Kay, Bruce E Herring

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