Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds

Biomaterials Research
Jin-Young ParkSeong-Ho Choi

Abstract

The aim of this study was to characterize the efficacy of nano-hydroxyapatite-coated silk fibroin constructs as a scaffold for bone tissue engineering and to determine the osteogenic effect of human dental pulp and periodontal ligament derived cells at an early stage of healing in rabbits. 3D silk fibroin constructs were developed and coated using nano-hydroxyapatite crystals. Dental pulp and periodontal ligament cells from extracted human third molars were cultured and seeded onto the silk scaffolds prior to in vivo implantation into 8 male New Zealand White rabbits. Four circular windows 8 mm in diameter were created in the calvarium of each animal. The defects were randomly allocated to the groups; (1) silk scaffold with dental pulp cells (DPSS), (2) silk scaffold with PDL cells (PDLSS), (3) normal saline-soaked silk scaffold (SS), and (4) empty control. The animals were sacrificed 2 (n = 4) or 4 weeks (n = 4) postoperatively. The characteristics of the silk scaffolds before and after cell seeding were analyzed using SEM. Samples were collected for histologic and histomorphometic analysis. ANOVA was used for statistical analysis. Histologic view of the experimental sites showed well-maintained structure of the silk scaffolds...Continue Reading

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Citations

Feb 24, 2017·The Journal of Craniofacial Surgery·María Pilar Pecci-LloretFrancisco Javier Rodríguez-Lozano
Feb 23, 2018·Tissue Engineering. Part C, Methods·Kang-Gon LeeBu-Kyu Lee
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Methods Mentioned

BETA
scanning electron microscopy

Software Mentioned

SPSS
Image
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