Regeneration of Solanum tuberosum Plants from Protoplasts Induces Widespread Genome Instability

Plant Physiology
Michelle FossiLuca Comai

Abstract

Nontransgenic genome editing in regenerable protoplasts, plant cells free of their cell wall, could revolutionize crop improvement because it reduces regulatory and technical complexity. However, plant tissue culture is known to engender frequent unwanted variation, termed somaclonal variation. To evaluate the contribution of large-scale genome instability to this phenomenon, we analyzed potatoes (Solanum tuberosum) regenerated from either protoplasts or stem explants for copy number changes by comparison of Illumina read depth. Whereas a control set of eight plants that had been propagated by cuttings displayed no changes, all 15 protoplast regenerants tested were affected by aneuploidy or structural chromosomal changes. Certain chromosomes displayed segmental deletions and duplications ranging from one to many. Resampling different leaves of the same plant found differences in three regenerants, indicating frequent persistence of instability. By comparison, 33 regenerants from stem explants used for Agrobacterium-mediated transformation displayed less frequent but still considerable (18%) large-scale copy number changes. Repetition of certain instability patterns suggested greater susceptibility in specific genomic sites. The...Continue Reading

Citations

Jun 2, 2020·Journal of Zhejiang University. Science. B·Yuan-Yuan TanQing-Yao Shu
May 19, 2019·Plant Cell Reports·Florian VeilletJean-Eric Chauvin
May 14, 2020·BMC Biotechnology·Zsófia BánfalviMihály Kondrák
Jan 5, 2021·Frontiers in Plant Science·Tom EeckhautJohan Van Huylenbroeck
Aug 20, 2019·Trends in Genetics : TIG·Luca Comai, Ek Han Tan
Feb 2, 2021·Frontiers in Plant Science·Satya Swathi Nadakuduti, Felix Enciso-Rodríguez
Feb 25, 2021·Plant Methods·Yeong Yeop JeongPil Joon Seo
Dec 29, 2020·Plant Communications·Florian VeilletJean-Luc Gallois
Apr 14, 2021·Transgenic Research·Jan G SchaartMarinus J M Smulders
Apr 20, 2021·Journal of Plant Physiology·Ravi B Anjanappa, Wilhelm Gruissem
Apr 27, 2021·Plant Communications·Yingxiao ZhangYiping Qi
Oct 30, 2021·Frontiers in Genome Editing·Jin-Jun YueChoun-Sea Lin
Jan 29, 2022·Plant Cell Reports·Chandler M MeyerPatrick J Krysan

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