Regeneration of the germline in the annelid Capitella teleta

Developmental Biology
Leah C Dannenberg, Elaine C Seaver

Abstract

The germline is essential for sexual reproduction and survival of the species. In many metazoans, the developmental potential to generate a distinct germline is segregated from somatic cell lineages early in embryogenesis, suggesting that the unique features of the germline must be established from its onset. Previous studies suggest that germ cells cannot regenerate once removed from the embryo, but few animals have been experimentally tested. We investigated the ability of the germline to regenerate in a lophotrochozoan, the segmented worm Capitella teleta, which has a stereotyped cell lineage program by deleting the germline precursor (cell 3D) in early stage embryos using an infrared laser. Larvae and juveniles resulting from germline deletions were examined for presence of multipotent progenitor cells (MPCs), stem cells that form the germ cells and somatic stem cells. In contrast to control deletions of a non-germline macromere, most larvae resulting from deletion of cell 3D lacked MPCs as assayed by expression of germline markers CapI-vasa, CapI-nanos and Ct-piwi1, but showed persistent expression of these markers in the somatic posterior growth zone. However, approximately 13% of experimental larvae had MPCs, indicative ...Continue Reading

Citations

May 24, 2020·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Gary M WesselNathalie Oulhen
Mar 20, 2021·The Science of the Total Environment·Amalia RosnerSébastien Cambier
Jun 7, 2021·Current Opinion in Cell Biology·Amelie A Raz, Yukiko M Yamashita
Oct 8, 2021·Biological Reviews of the Cambridge Philosophical Society·Baruch RinkevichBert Hobmayer

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