PMID: 9159621May 1, 1997Paper

Regenerative healing of incisional wounds in midgestational murine hearts in organ culture

The Journal of Thoracic and Cardiovascular Surgery
C J BlewettT M Krummel

Abstract

Using an organ-culture fetal heart repair model, we explored fetal repair in tissues other than dermis. Wounded fetal mouse hearts of 14 and 18 days' gestation (term = 20 days), as well as hearts of 22 days' gestation (newborn), were maintained in serum-free medium. Specimens were fixed at 2, 7, and 11 days and then processed for histologic examination. Small fragments of fetal hearts from all time points were cultured as explants. The migration of cells from the periphery of the explants was compared at day 4, and the pattern of microfilaments in these cells was assessed. In 14-day hearts (n = 18), tissue architecture was rapidly reestablished without an inflammatory response or scarring, constituting regenerative repair. In 18-day hearts (n = 18), no reestablishment of muscle fibers or wound closure occurred. In the 22-day explants (n = 12) the wounds closed by scarring. Cell migration from 14-day explants was 4.7 +/- 2.3 ocular units; from 18-day explants, it was 2.6 +/- 1.1 ocular units; and from 22-day explants, it was 0.9 +/- 0.4 ocular units. Microfilaments of 14-day cells were arranged at the periphery of the cell consistent with cardiomyocytes. Microfilaments of 18- and 22-day cells were arranged in parallel arrays (st...Continue Reading

References

Apr 1, 1991·The Journal of Surgical Research·M T LongakerN S Adzick
Jan 1, 1990·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·J H HaynesR F Diegelmann
Oct 1, 1971·Journal of Pediatric Surgery·J D Burrington
Jul 1, 1995·Journal of Pediatric Surgery·C J BlewettT M Krummel
Oct 1, 1993·Journal of Pediatric Surgery·J C BleacherT M Krummel

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Citations

Feb 4, 2010·Journal of Anesthesia·Pei-Jung ChangYu-Chuan Tsai
May 24, 2007·Tissue Engineering·Ioannis V YannasMichael T Longaker
Sep 27, 2002·Seminars in Cell & Developmental Biology·John M Leferovich, Ellen Heber-Katz
Feb 4, 2009·The Journal of Cell Biology·Simonetta Ausoni, Saverio Sartore
Oct 18, 2005·The Journal of Pediatrics·Audrey C MarshallJames E Lock
Jan 30, 2015·Journal of the American Heart Association·Tal KonfinoJonathan Leor
Jan 19, 2007·American Journal of Physiology. Cell Physiology·W A LaFramboiseM J Becich
Aug 12, 1998·The Journal of Surgical Research·J A IoconoH P Ehrlich

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