Region-based multifocus image fusion for the precise acquisition of Pap smear images

Journal of Biomedical Optics
Santiago Tello-Mijares, Jesús Bescós

Abstract

A multifocus image fusion method to obtain a single focused image from a sequence of microscopic high-magnification Papanicolau source (Pap smear) images is presented. These images, captured each in a different position of the microscope lens, frequently show partially focused cells or parts of cells, which makes them unpractical for the direct application of image analysis techniques. The proposed method obtains a focused image with a high preservation of original pixels information while achieving a negligible visibility of the fusion artifacts. The method starts by identifying the best-focused image of the sequence; then, it performs a mean-shift segmentation over this image; the focus level of the segmented regions is evaluated in all the images of the sequence, and best-focused regions are merged in a single combined image; finally, this image is processed with an adaptive artifact removal process. The combination of a region-oriented approach, instead of block-based approaches, and a minimum modification of the value of focused pixels in the original images achieve a highly contrasted image with no visible artifacts, which makes this method especially convenient for the medical imaging domain. The proposed method is compa...Continue Reading

References

Feb 5, 1998·Journal of Microscopy·A SantosF del Pozo
Sep 21, 2004·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Zhou WangEero P Simoncelli
Dec 18, 2004·Microscopy Research and Technique·Yu SunBradley J Nelson
Oct 20, 2007·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·X Y LiuY Sun
Aug 13, 2001·Optics Express·Q GuihongY Pingfan
Dec 24, 2011·Journal of Biomedical Optics·Lingbo KongYong-qing Li
Mar 14, 2013·Optics Express·Long ChenC L Philip Chen
Apr 2, 2016·Computational and Mathematical Methods in Medicine·Santiago Tello-Mijares, Francisco Flores

❮ Previous
Next ❯

Citations

Jul 6, 2021·Computational and Mathematical Methods in Medicine·Yongxin ZhangPeng Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.