Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue

Scientific Reports
Yuka YajimaTatsuaki Tsuruyama

Abstract

Matrix-assisted laser desorption ionization image mass spectrometry (MALDI-IMS) has been developed for the identification of peptides in various tissues. The MALDI-IMS signal distribution patterns and quantification of the signal intensities of the regions of interest (ROI) with healthy regions were compared for identification of the disease specific biomarkers. We performed a new ROI analysis using the conventional t-test and data number independent Cohen's d-value analysis. Using these techniques, we analysed heart tissues after acute myocardial infarction (AMI). As a result, IMS signals of mitochondrial adenosine triphosphate synthase alpha subunit (ATP5A), myosin-6/7(MYH6/7), aortic actin, and the myosin light chain 3 (MYL3) were identified in the infarcted region. In particular, the signals of MYH7 are significantly greater in the infarcted region using ROI analysis. ROI analysis using MALDI-IMS may be a promising technique for the identification of biomarkers for pathological studies that involve the comparison of diseased and control areas.

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Citations

Sep 15, 2019·The Plant Journal : for Cell and Molecular Biology·Philippe FuchsMarkus Schwarzländer
Apr 14, 2019·Analytical and Bioanalytical Chemistry·Stephanie T P MezgerRon M A Heeren
Mar 6, 2021·Scientific Reports·Aleksandra Aljakna KhanSara Sabatasso
Jul 28, 2020·Current Protein & Peptide Science·Madhuri JayathirthaCostel C Darie

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Methods Mentioned

BETA
ELISA

Software Mentioned

Imaging MS Solution®
Protein Pilot
Proteomic System Performance Evaluation Pipeline ( PSPEP )
Mascot
Protein Quantitation 1 . 0 MicroApp ( PQMA )
ProteinPilot
Imaging MS Solution
PQMA
Peak View

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