Region-specific expression and secretion of the fibrinogen-related protein, fgl2, by epithelial cells of the hamster epididymis and its role in disposal of defective spermatozoa.

The Journal of Biological Chemistry
Gary E OlsonMichael H Melner

Abstract

The cauda epididymidis functions in the storage and protection of mature, fertile spermatozoa. We previously identified a region-specific secretory glycoprotein (termed HEP64) of the hamster proximal cauda epididymidis that specifically bound and coated the nonviable, but not the viable, spermatozoa within the epididymal lumen. In this study we employed expression screening of a hamster epididymal cDNA library to obtain the full-length sequence of HEP64 and to identify it as the fibrinogen-like protein fgl2. Northern blot analysis demonstrated that fgl2 mRNA is highly expressed by the proximal cauda epididymidis in comparison to other hamster tissues examined, and, in situ hybridization analysis of the epididymis revealed that fgl2 mRNA exhibited a region- and principal cell-specific expression pattern. Immunohistochemistry confirmed the association of fgl2 with abnormal spermatozoa in the cauda epididymidis and revealed smaller fgl2-containing particles. Immunoelectron microscopy revealed that fgl2 was distributed throughout an amorphous, "death cocoon," complex assembled onto abnormal spermatozoa and that the smaller fgl2 aggregates consisted of the amorphous material with embedded sperm fragments, organelles, and membrane ve...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1992·Protein Science : a Publication of the Protein Society·R F Doolittle
Dec 1, 1988·Biology of Reproduction·G E OlsonG R Davenport
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T KoyamaH Saito
Jun 1, 1969·Biology of Reproduction·J Martan
Oct 1, 1994·International Journal of Andrology·R WeissenbergL M Lewin
Jan 1, 1995·Microscopy Research and Technique·B T Hinton, M A Palladino
Jan 1, 1997·Nucleic Acids Research·J E HansenS Brunak
Jan 1, 1996·Current Topics in Developmental Biology·B T HintonJ C Labus
Sep 18, 1998·Andrologia·C KirchhoffS Schröter
Dec 10, 1998·Nucleic Acids Research·K HofmannA Bairoch
Apr 9, 1999·International Journal of Andrology·R C Jones
Apr 20, 1999·International Review of Cytology·C Kirchhoff
Jul 20, 1999·Molecular and Cellular Endocrinology·R Bailey, M D Griswold
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·M Leist, M Jäättelä
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·P M HensonV A Fadok
Aug 22, 2001·Biology of Reproduction·K M Jervis, B Robaire
Sep 14, 2001·American Journal of Reproductive Immunology : AJRI·M KnackstedtD A Clark
May 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Camie W Y ChanGary A Levy
May 30, 2002·Nature Reviews. Immunology·Teizo Fujita
Jan 14, 2003·Annual Review of Immunology·Uffe HolmskovJens C Jensenius
Mar 22, 2003·Current Opinion in Cell Biology·Rubén Claudio Aguilar, Beverly Wendland
Apr 3, 2003·Microscopy Research and Technique·Gary E OlsonSubir K Nagdas
Apr 12, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Camie W Y ChanGary A Levy
Jun 27, 2003·Trends in Biochemical Sciences·David C Schwartz, Mark Hochstrasser
Apr 8, 1961·Nature·T D GLOVER
Feb 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wayne W HancockStephen T Smiley

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Citations

Sep 24, 2015·Journal of Cellular Physiology·Olivier D'AmoursRobert Sullivan
Nov 11, 2008·European Journal of Immunology·Hao LiuGary A Levy
Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·P Sutovsky
Mar 26, 2013·PloS One·Tanja KögelHans-Hermann Gerdes
Sep 19, 2014·Biology of Reproduction·John F OdhiamboPeter Sutovsky
Dec 8, 2007·Journal of Cellular Physiology·Kathleen M BaskaPeter Sutovsky
Feb 23, 2007·The Journal of Biological Chemistry·Gary E OlsonRaymond F Burk
Sep 5, 2020·American Journal of Reproductive Immunology : AJRI·Cottrell T TamessarJohn E Schjenken
Nov 17, 2007·Journal of Proteome Research·A Stephen GeorgiouAlireza Fazeli

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