PMID: 1194809Sep 1, 1975Paper

Region-specific immunoassays for parathyroid hormone

The Journal of Endocrinology
P M BarlingJ L O'Riordan

Abstract

Immunoassays specific for limited regions of bovine parathyroid hormone were developed in four ways. With the heterogeneous antisera produced by immunizing with intact bovine parathyroid hormone (BPTH 1-84), the specificity of radioimmunoassays could be enhanced by presaturating either with an amino-terminal (BPTH 1-34) or carboxy-terminal (BPTH 53-84) fragment. Then, the antibodies which had not been neutralized reacted exclusively with the opposite end of the molecule, even using [125I]BPTH 1-84 as tracer. With some antisera, the appropriate fragment and intact hormone reacted identically. However, with other antisera, the fragment reacted less well than the intact hormone, possibly because these antisera contain antibodies reacting with the middle of the molcule. Using the labelled fragment ([125I]BPTH 1-34) as tracer, with heterogeneous antisera, radioimmunoassays specific for the amino-terminal region were obtained. With one antiserum, BPTH 1.34 reached identically with the intact hormone, but with another antiserum, the fragment was more reactive than the intact molecule. A region-specific radioimmunoassay was also developed using antibodies produced by immunization with a fragment of the hormone. An antiserum raised agai...Continue Reading

Citations

Jul 3, 1979·Calcified Tissue International·A ChantraineP Franchimont
Sep 1, 1979·Scandinavian Journal of Clinical and Laboratory Investigation·K M GautvikR Tollman
Dec 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J J BrookmanG N Hendy
Sep 1, 1977·Clinical Endocrinology·S E PapapoulosJ L O'riordan
Feb 1, 1979·Clinical Endocrinology·J A KanisG Heynen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Related Papers

Clinical Endocrinology
G N Hendy, J L O'Riordan
Clinica Chimica Acta; International Journal of Clinical Chemistry
J Woo, F R Singer
© 2022 Meta ULC. All rights reserved