PMID: 9427503Jan 14, 1998Paper

Regional, developmental and interspecies expression of the four NMDAR2 subunits, examined using monoclonal antibodies

Brain Research. Molecular Brain Research
D J LaurieP H Seeburg

Abstract

Mouse monoclonal antibodies were raised against bacterially expressed protein sequences of the NR2A, NR2B, NR2C and NR2D subunits of the rat NMDA receptor. From immunoblots of rat brain proteins, the apparent molecular weights of these subunits were 165, 170, 135 and 145 kDa, respectively. Proteins of similar masses were observed on immunoblots of specifically transfected HEK293 cells. Deglycosylation with endoglycosidase F reduced the mass of each endogenous NR2 subunit by approximately 10 kDa. In distribution studies, NR2A-immunoreactive protein (IRP) was located throughout the adult rat brain, NR2B-IRP was primarily in the forebrain, NR2C-IRP was predominantly in the cerebellum and NR2D-IRP was mainly found in the thalamus, midbrain and brainstem. Whereas NR2A- and NR2C-IRPs increased during rat brain post-natal development, NR2B- and NR2D-IRPs were abundant at birth and declined with age, especially in cerebellum. NR2-IRPs of mouse, rabbit, frog and human brain were of sizes similar to those of the corresponding rat subunits and were similarly distributed. In summary, NR2 subunits are large glycoproteins whose specific expression profiles in the brain are developmentally and regionally regulated and which are similarly expr...Continue Reading

References

Jun 30, 1992·Biochemical and Biophysical Research Communications·H SugiharaS Nakanishi
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R G KalbS Hockfield
Jul 2, 1992·Nature·T KutsuwadaM Arakawa
Mar 24, 1992·European Journal of Pharmacology·K OkakuraH Wada
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G M DurandR S Zukin
Mar 23, 1992·FEBS Letters·M YamazakiM Mishina
Nov 7, 1991·Nature·K MoriyoshiS Nakanishi
Aug 15, 1989·European Journal of Pharmacology·T HonoréM Nielsen
Aug 1, 1995·Brain Research. Molecular Brain Research·D J LaurieT R Tölle
Aug 16, 1994·European Journal of Pharmacology·D J Laurie, P H Seeburg
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R Planells-CasesM Montal
Sep 1, 1994·Brain Research. Molecular Brain Research·M A RivaG Racagni
Feb 1, 1995·Journal of Neurochemistry·J ZhongP B Molinoff
Oct 22, 1994·The Journal of Comparative Neurology·E HartveitH Wässle
Jul 1, 1994·Physiological Reviews·C J McBain, M L Mayer
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·H LiuA I Basbaum
Dec 1, 1993·Protein Science : a Publication of the Protein Society·A K KöpkeJ Spiess
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G M DurandR S Zukin
Jun 28, 1996·The Journal of Biological Chemistry·J Blahos, R J Wenthold

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Citations

Feb 15, 2001·Journal of Biomedical Science·K M Davis, J Y Wu
May 12, 2012·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Mark BandAaron Avivi
Oct 1, 2009·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Long-Jun Wu, Min Zhuo
Sep 5, 1998·Brain Research·N YaghoubiD H Farb
Sep 11, 1999·Trends in Neurosciences·F Conti, R J Weinberg
Jul 16, 2002·Brain Research. Molecular Brain Research·Hosein K KiaDonald W Pfaff
Dec 4, 2003·Neurochemistry International·Mohammad Tabish, Maharaj K Ticku
Oct 9, 1999·Neuroscience Letters·R C Klein, F J Castellino
Feb 7, 2001·Current Opinion in Neurobiology·J Z Tsien
Dec 15, 2004·Visual Neuroscience·Michael KalloniatisHeinz Wässle
Jun 20, 2001·Journal of Neurochemistry·N Janssens, A S Lesage
Oct 24, 2006·Genes, Brain, and Behavior·M A De Souza SilvaE Dere
Jul 22, 1999·Annals of the New York Academy of Sciences·E B Ziff
Mar 1, 2012·PloS One·Bethany L PetersonChristopher P Fall
Aug 8, 2012·PloS One·Maile A HensonBenjamin D Philpot
Oct 2, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hongjie YuanStephen F Traynelis
Dec 14, 1999·The European Journal of Neuroscience·G NaseH Monyer
Mar 19, 2014·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Min LiWenwen Cheng
Jun 10, 2011·Genes and Immunity·UNKNOWN International Multiple Sclerosis Genetics Consortium

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