Regional difference in stainability with calcium-sensitive acetoxymethyl-ester probes in mouse brain slices

The International Journal of Neuroscience
Shigehiro NamikiYuji Ikegaya

Abstract

Loading neurons with membrane permeable Ca2+ indicators is a core experimental procedure in functional multineuron Ca2+ imaging (fMCI), an optical technique for monitoring multiple neuronal activities. Although fMCI has been applied to several brain networks, including cerebral cortex, hippocampus, and cerebellum, no studies have systematically addressed the dye-loading efficiency in different brain regions. Here, we describe the stainability of Oregon Green 488 BAPTA-1AM in mouse acute brain slice preparations. The data are suggestive of the potential usability of fMCI in many brain regions, including olfactory bulb, thalamus, dentate gyrus, habenular nucleus, and pons.

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Citations

Jan 17, 2016·Molecular Brain·Shin-ichiro HoriganeHaruhiko Bito
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sarah Feldt MuldoonRosa Cossart
Apr 24, 2009·Journal of Neurophysiology·Takuya SasakiYuji Ikegaya
Jul 8, 2011·Journal of Neurophysiology·Romain FranconvilleIsabel Llano
Jul 16, 2009·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Mika Mizunuma, Yuji Ikegaya

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