Regional differences in L-selectin expression in murine intestinal lymphocytes

Gastroenterology
F SeiboldC O Elson

Abstract

The expression of the lymphocyte homing receptor and activation marker L-selectin is different in colon and small intestinal intraepithelial lymphocytes (IELs). In this study, the mechanism of this difference in L-selectin expression was investigated. L-selectin expression on lymphocytes was measured by flow cytometry. L-selectin messenger RNA (mRNA) was detected by reverse-transcription polymerase chain reaction. L-Selectin expression on peripheral lymphocytes was analyzed after incubation with cytokines, food and bacterial antigens, and homogenates of small and large bowel. L-selectin was expressed by none of the small intestinal IELs but by 30% of those in the colon and by 60% of splenocytes. mRNA for L-selectin was detectable in isolated lymphocytes of all three sites. L-Selectin was down-regulated in colon IELs during colitis and up-regulated in small intestinal IELs after in vitro culture for 48 hours. Incubation of splenocytes with small intestinal homogenates led to a rapid down-regulation of L-selectin (1% vs. 60% untreated). Preincubation with a metalloproteinase inhibitor prevented L-selectin loss. The mechanism of the differential expression of L-selectin in mouse small intestine and colon appears to be an increased...Continue Reading

Citations

Mar 3, 2009·Immunologic Research·Elizabeth W SorensenEdith M Lord
Jul 19, 2005·Digestion·Frank Seibold
Jun 28, 2007·European Journal of Immunology·Sanda StankovicLeonard C Harrison
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sun-Young ChangMi-Na Kweon
Oct 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chikako WatanabeHiromasa Ishii

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