Jan 16, 2014

Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts

PloS One
Gopal SivagangabalanKumaraswamy Nanthakumar

Abstract

Structural differences between ventricular regions may not be the sole determinant of local ventricular fibrillation (VF) dynamics and molecular remodeling may play a role. To define regional ion channel expression in myopathic hearts compared to normal hearts, and correlate expression to regional VF dynamics. High throughput real-time RT-PCR was used to quantify the expression patterns of 84 ion-channel, calcium cycling, connexin and related gene transcripts from sites in the LV, septum, and RV in 8 patients undergoing transplantation. An additional eight non-diseased donor human hearts served as controls. To relate local ion channel expression change to VF dynamics localized VF mapping was performed on the explanted myopathic hearts right adjacent to sampled regions. Compared to non-diseased ventricles, significant differences (p<0.05) were identified in the expression of 23 genes in the myopathic LV and 32 genes in the myopathic RV. Within the myopathic hearts significant regional (LV vs septum vs RV) expression differences were observed for 13 subunits: Nav1.1, Cx43, Ca3.1, Cavα2δ2, Cavβ2, HCN2, Na/K ATPase-1, CASQ1, CASQ2, RYR2, Kir2.3, Kir3.4, SUR2 (p<0.05). In a subset of genes we demonstrated differences in protein expr...Continue Reading

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  • Citations9

Citations

Mentioned in this Paper

KCNJ2 gene
Cardiomyopathy, Familial Idiopathic
Ischemia
Channel Activity
In Silico
Ventricular Fibrillation
KCNJ8 gene
KCNJ4 gene
Calcium [EPC]
KCNJ5 wt Allele

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