PMID: 8614566Nov 17, 1995Paper

Regional quantification of dopamine transporter mRNA in rat brain using a ribonuclease protection assay

Neuroscience Letters
N M RichtandRonald Kuczenski

Abstract

We describe the regional distribution of dopamine transporter (DAT) mRNA in selected regions of rat brain using a highly sensitive and specific nuclease protection assay. This method determines the absolute quantity of mRNA expressed in the brain regions surveyed. DAT mRNA level varied widely between brain regions, and was detected only in cell body regions of the major dopaminergic pathways. Highest expression was seen in substantia nigra/ventral tegmentum (SN/VTA). Lower but detectable expression of a protected mRNA of the expected size was observed within hypothalamus. Expression could not be detected by this method in other brain regions studied. Our results indicate that this method is sufficiently sensitive to allow study of mRNA expression in individual animals.

References

May 1, 1992·Brain Research. Molecular Brain Research·S ShimadaG Uhl
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T B UsdinB J Hoffman
Jan 1, 1990·Progress in Neurobiology·A S Horn
Nov 4, 1988·Science·G F Koob, F E Bloom
Jul 1, 1987·British Journal of Addiction·J J O'Connor, S Stafford-Johnson
Apr 1, 1971·British Journal of Pharmacology·L L Iversen
Mar 1, 1974·Pharmacology, Biochemistry, and Behavior·D S Segal, A J Mandell
Sep 1, 1994·Pharmacology, Biochemistry, and Behavior·C W SchindlerS R Goldberg
Dec 1, 1993·Brain Research. Molecular Brain Research·S J AugoodP C Emson
Mar 1, 1994·Brain Research. Molecular Brain Research·C CerrutiM J Kuhar
Oct 1, 1993·Neuroendocrinology·B Meister, R Elde
Aug 1, 1993·Brain Research. Molecular Brain Research·M FujitaM Tohyama
Apr 1, 1993·Brain Research. Molecular Brain Research·C CerrutiG R Uhl
Oct 1, 1995·Brain Research. Molecular Brain Research·N M RichtandR Kuczenski

❮ Previous
Next ❯

Citations

May 19, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Annabelle M BelcherJohn F Marshall
Jul 20, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Annabelle M BelcherJohn F Marshall
Nov 26, 2011·Neuropharmacology·Carmela M ReichelRonald E See

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.