Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle.

The Journal of Cell Biology
Ge YangGaudenz Danuser

Abstract

Continuous poleward movement of tubulin is a hallmark of metaphase spindle dynamics in higher eukaryotic cells and is essential for stable spindle architecture and reliable chromosome segregation. We use quantitative fluorescent speckle microscopy to map with high resolution the spatial organization of microtubule flux in Xenopus laevis egg extract meiotic spindles. We find that the flux velocity decreases near spindle poles by approximately 20%. The regional variation is independent of functional kinetochores and centrosomes and is suppressed by inhibition of dynein/dynactin, kinesin-5, or both. Statistical analysis reveals that tubulin flows in two distinct velocity modes. We propose an association of these modes with two architecturally distinct yet spatially overlapping and dynamically cross-linked arrays of microtubules: focused polar microtubule arrays of a uniform polarity and slower flux velocities are interconnected by a dense barrel-like microtubule array of antiparallel polarities and faster flux velocities.

References

Jan 1, 1991·Methods in Enzymology·A HymanT Mitchison
Aug 11, 2004·The Journal of Cell Biology·Neil J Ganem, Duane A Compton
Aug 18, 2004·The Journal of Cell Biology·Jedidiah Gaetz, Tarun M Kapoor
Nov 9, 2004·Current Biology : CB·Mimi Shirasu-HizaTimothy J Mitchison
Dec 8, 2004·The Journal of Cell Biology·David T MiyamotoTimothy J Mitchison
Mar 15, 2005·Journal of Cell Science·Gregory C RogersDavid J Sharp
May 6, 2005·Nature·Lukas C KapiteinChristoph F Schmidt
May 18, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T J Mitchison
Oct 26, 2005·Current Biology : CB·Neil J GanemDuane A Compton
Apr 26, 2006·The Journal of Cell Biology·Lisa A CameronE D Salmon
Nov 8, 2006·The Journal of Cell Biology·Kendra S BurbankTimothy J Mitchison
Dec 19, 2006·Current Opinion in Cell Biology·Benjamin H Kwok, Tarun M Kapoor
Apr 25, 2007·The Journal of Cell Biology·Dong ZhangDavid J Sharp
May 19, 2007·Current Biology : CB·Ryoma OhiTimothy J Mitchison
Jun 8, 2007·Molecular Biology of the Cell·Daniel W BusterDavid J Sharp
Aug 19, 2007·Current Biology : CB·Kendra S BurbankDaniel S Fisher
Oct 16, 2007·Nature Cell Biology·Ge YangTarun M Kapoor

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Citations

Feb 11, 2012·Quarterly Reviews of Biophysics·J Richard McIntoshFazly I Ataullakhanov
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Benjamin R HoughtalingTarun M Kapoor
May 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Agnieszka KolanoMarie-Hélène Verlhac
Sep 10, 2010·The Journal of Biological Chemistry·Benjamin GoodmanYixian Zheng
Jan 30, 2010·Molecular Biology of the Cell·Nan MaPatricia Wadsworth
Feb 18, 2012·Molecular Biology of the Cell·Alyssa GablePatricia Wadsworth
Aug 25, 2012·Molecular Biology of the Cell·Ting-Yu YehTrina A Schroer
Jan 5, 2012·PLoS Biology·David HalpinRebecca Heald
Nov 1, 2011·Molecular Biology of the Cell·J Richard McIntosh
Jul 8, 2010·Annual Review of Cell and Developmental Biology·Gohta Goshima, Jonathan M Scholey
Jul 30, 2014·The Journal of Cell Biology·Kara J Helmke, Rebecca Heald
Feb 24, 2010·The Journal of Cell Biology·Jesse C GatlinEdward D Salmon
Dec 22, 2010·The Journal of Cell Biology·Rose LoughlinFrançois Nédélec
Jul 16, 2014·The Journal of Cell Biology·Mary Williard EltingSophie Dumont
Jul 8, 2009·The Journal of Cell Biology·Irina MatosHelder Maiato
Jan 20, 2016·The Journal of Cell Biology·António J PereiraHelder Maiato
Apr 22, 2010·Experimental Cell Research·James I LimGaudenz Danuser
Dec 22, 2009·Current Opinion in Cell Biology·Gohta Goshima, Akatsuki Kimura
Feb 15, 2013·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Ge Yang
Jun 26, 2012·Cytoskeleton·X Johné Liu
Aug 21, 2013·Gene·Edward J WojcikSunyoung Kim
May 1, 2012·Cell·Jan BruguésDaniel J Needleman
Jan 27, 2009·Current Biology : CB·Zhang-Yi LiangSharyn Anne Endow
Dec 26, 2008·Current Biology : CB·Linda Amos
Dec 25, 2012·Biophysical Journal·Elizabeth A Booth-GauthierKris N Dahl
Mar 5, 2016·Molecular Biology of the Cell·Hailing ZongStephanie C Ems-McClung
Nov 12, 2016·Journal of Biomechanics·Travis J ArmigerKris Noel Dahl
Aug 24, 2013·Biology of Reproduction·Katie Howe, Greg FitzHarris
Jul 1, 2014·Nature Cell Biology·Nicolas Lecland, Jens Lüders
Mar 13, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Stephen T Spagnol, Kris Noel Dahl
Jan 15, 2016·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Tong GaoMichael J Shelley
Aug 8, 2020·Biology of the Cell·Gabriel Guilloux, Romain Gibeaux
Apr 14, 2017·Cytoskeleton·Paul MooneyJesse C Gatlin
Dec 1, 2013·Cellular and Molecular Bioengineering·M D Betterton, J Richard McIntosh
Apr 24, 2020·Nature Physics·Sebastian FürthauerMichael J Shelley
Oct 11, 2017·Annual Review of Cell and Developmental Biology·J Richard McIntosh
May 12, 2018·Journal of Cell Science·Travis J ArmigerKris Noel Dahl
May 23, 2019·The Journal of Cell Biology·Ana F DavidDaniel W Gerlich
Dec 18, 2019·Biophysical Journal·Moritz StriebelErwin Frey
Apr 15, 2021·Seminars in Cell & Developmental Biology·Kruno Vukušić, Iva M Tolić
May 4, 2011·Cold Spring Harbor Protocols·Lisa A CameronGe Yang

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Methods Mentioned

BETA
fluorescent
speckle microscopy
fluorescence microscopy
confocal microscopy

Software Mentioned

MetaMorph
R
Nortest
MCLUST

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