Regionally Discrete Aqueous Humor Outflow Quantification Using Fluorescein Canalograms

PloS One
Ralitsa T LoewenNils A Loewen

Abstract

To visualize and quantify conventional outflow directly in its anatomic location. We obtained fluorescein canalograms in six porcine whole eyes and six porcine anterior segment cultures. Eyes were perfused with a constant pressure of 15 mmHg using media containing 0.017 mg/ml fluorescein. Flow patterns were visualized using a stereo dissecting microscope equipped for fluorescent imaging. Images were captured every 30 seconds for 20 minutes for time lapse analysis. Anterior chamber cultures were imaged again on day three of culture. Canalograms were first analyzed for filling time per quadrant. We then wrote a program to automatically compute focal flow fits for each macropixel and to detect convergent perilimbal flow patterns with macropixels grouped into 3 equal-radial width rings around the cornea. A generalized additive model was used to determine fluorescence changes of individual macropixels. The resulting imaging algorithm deployed 1024 macropixels that were fit to determine maximum intensity and time to fill. These individual fits highlighted the focal flow function. In whole eyes, significantly faster flow was seen in the inferonasal (IN) and superonasal (SN) quadrants compared to the superotemporal (ST) and inferotempo...Continue Reading

References

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Citations

Apr 5, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ying HongNils A Loewen
Mar 19, 2019·Current Opinion in Ophthalmology·Sayoko E MoroiJulia E Richards
Mar 29, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Chao WangNils A Loewen
Sep 9, 2017·International Ophthalmology Clinics·Tavé van Zyl, Angela Turalba
May 23, 2017·F1000Research·Katherine FallanoNils A Loewen
Apr 5, 2018·Scientific Reports·Yalong DangNils A Loewen
May 31, 2018·Investigative Ophthalmology & Visual Science·Susannah WaxmanNils A Loewen
Nov 21, 2018·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yalong DangNils A Loewen
Aug 22, 2020·Translational Vision Science & Technology·Chen XinNingli Wang
May 4, 2018·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yalong DangNils A Loewen
Jan 27, 2019·Investigative Ophthalmology & Visual Science·Hanna M GottschalkChristian van Oterendorp
Oct 17, 2019·Translational Vision Science & Technology·Kevin C SnyderGillian J McLellan

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Anterior Chamber of Eye Structure
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