Jan 1, 1989

Regionally selective alterations in enzymatic activities and metabolic fluxes during thiamin deficiency

Neurochemical Research
Gary E GibsonJ P Blass


To further elucidate the molecular basis of the selective damage to various brain regions by thiamin deficiency, changes in enzymatic activities were compared to carbohydrate flux through various pathways from vulnerable (mammillary bodies and inferior colliculi) and nonvulnerable (cochlear nuclei) regions after 11 or 14 days of pyrithiamin-induced thiamin deficiency. After 11 days, large decreases (-43 to -59%) in transketolase (TK) occurred in all 3 regions; 2-ketoglutarate dehydrogenase (KGDHC) declined (-45%), but only in mammillary bodies; pyruvate dehydrogenase (PDHC) was unaffected. By day 14, TK remained reduced by 58%-66%; KGDHC was now reduced in all regions (-48 to -55%); PDHC was also reduced (-32%), but only in the mammillary bodies. Thus, the enzyme changes did not parallel the pathological vulnerability of these regions to thiamin deficiency. 14CO2 production from 14C-glucose labeled in various positions was utilized to assess metabolic flux. After 14 days, CO2 production in the vulnerable regions declined severely (-46 to 70%) and approximately twice as much as those in the cochlear nucleus. Also by day 14, the ratio of enzymatic activity to metabolic flux increased as much as 56% in the vulnerable regions, but ...Continue Reading

  • References18
  • Citations16


  • References18
  • Citations16


Mentioned in this Paper

Mammillary Body Structure
Metabolic Process, Cellular
Biochemical Pathway
Thiamine Deficiency
Enzymes, antithrombotic
Pyruvate dehydrogenase
August Rats
Glucose, (beta-D)-Isomer
Cochlear Nucleus Structure

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