Regionally selective alterations in enzymatic activities and metabolic fluxes during thiamin deficiency

Neurochemical Research
G E GibsonJ Blass

Abstract

To further elucidate the molecular basis of the selective damage to various brain regions by thiamin deficiency, changes in enzymatic activities were compared to carbohydrate flux through various pathways from vulnerable (mammillary bodies and inferior colliculi) and nonvulnerable (cochlear nuclei) regions after 11 or 14 days of pyrithiamin-induced thiamin deficiency. After 11 days, large decreases (-43 to -59%) in transketolase (TK) occurred in all 3 regions; 2-ketoglutarate dehydrogenase (KGDHC) declined (-45%), but only in mammillary bodies; pyruvate dehydrogenase (PDHC) was unaffected. By day 14, TK remained reduced by 58%-66%; KGDHC was now reduced in all regions (-48 to -55%); PDHC was also reduced (-32%), but only in the mammillary bodies. Thus, the enzyme changes did not parallel the pathological vulnerability of these regions to thiamin deficiency. 14CO2 production from 14C-glucose labeled in various positions was utilized to assess metabolic flux. After 14 days, CO2 production in the vulnerable regions declined severely (-46 to 70%) and approximately twice as much as those in the cochlear nucleus. Also by day 14, the ratio of enzymatic activity to metabolic flux increased as much as 56% in the vulnerable regions, but ...Continue Reading

References

Dec 22, 1977·The New England Journal of Medicine·J P Blass, G E Gibson
Aug 1, 1976·The Journal of Nutrition·D W McCandlessC E Cassidy
Oct 1, 1968·The Journal of Clinical Investigation·D W McCandlessM Cook
Jan 1, 1982·Annals of the New York Academy of Sciences·G GibsonJ Blass
Sep 1, 1982·Journal of Neurochemistry·J P BlassA Baker
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Jun 1, 1981·Archives of Neurology·J C TroncosoD L Price
Jan 1, 1965·Journal of Neuropathology and Experimental Neurology·P M DREYFUS

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Citations

Sep 1, 1994·Metabolic Brain Disease·L Bettendorff
Mar 1, 1995·Metabolic Brain Disease·P R MartinC K Singleton
Feb 17, 2000·Neurochemistry International·G E GibsonN Y Calingasan
Jan 30, 1999·The International Journal of Biochemistry & Cell Biology·G SchenkP F Nixon
Sep 21, 2010·Neurochemistry International·Agnieszka Jankowska-KulawyAndrzej Szutowicz
Jul 24, 2010·Journal of Neurochemistry·Agnieszka Jankowska-KulawyAndrzej Szutowicz
Sep 1, 2004·Neurochemistry International·Parvesh BubberGary E Gibson
Dec 14, 2016·Scientific Reports·Lennart BalkTomas Hansson
Aug 1, 1995·Molecular Medicine Today·J N DavisJ C Chisholm
Aug 15, 1994·The Journal of Comparative Neurology·N Y CalingasanG E Gibson
Dec 29, 2020·Frontiers in Systems Neuroscience·Tanuja Bordia, Natalie M Zahr
Jan 1, 1992·Research in Veterinary Science·A A OlkowskiD A Christensen

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