Regionally specific distribution of the binding of anti-glutamine synthetase and anti-S100 antibodies and of Datura stramonium lectin in glial domains of the optic lobe of the giant prawn

Glia
Silvana AllodiLeny A Cavalcante

Abstract

We previously characterized some crustacean glial cells by markers such as 2',3'-cyclic nucleotide 3'-phosphodiesterase and glial fibrillary acidic protein. Here we use antibodies against glutamine synthetase full-length molecule (anti-GS/FL), a GS C-terminal peptide (anti-GS/20aa-C), and brain S100 (anti-S100), as well as the binding of the insect glia and rat astrocytic marker Datura stramonium lectin (DSL), in the optic lobe of the prawn Macrobrachium rosenbergii. All markers label the lamina ganglionaris cartridge region (lighter: anti-GS/FL; heavier: DSL). In addition, anti-GS/FL labels superficial somata of external and internal medullas and internal chiasm cells. Both anti-GS/20aa-C and anti-S100 label heavily the glial sheaths of the lamina ganglionaris. In addition, anti-S100 binds to the perineurial glia of medullary parenchymal vessels. Western blot analyses show that both anti-GS/FL and anti-GS/20aa-C bind mostly to a band of 50-55 kDa, compatible with a long isoform of vertebrate GS, and accessorily to a possible dimer and, in the case of anti-GS/20aa-C, to an ill-defined band of intermediate mass. Binding of anti-S100 is selective for a single band of about 68 kDa but shows no protein in the weight range of the ca...Continue Reading

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Citations

Jan 10, 2008·Development Genes and Evolution·Cintia Carla NivaMaroko Myohara
Jun 23, 2012·PloS One·Paula Grazielle Chaves da SilvaSilvana Allodi
Jan 19, 2016·Cytotechnology·Inês Júlia Ribas WajsenzonSilvana Allodi
Nov 23, 2006·The Journal of Comparative Neurology·Jeremy M SullivanBarbara S Beltz
May 18, 2011·Glia·Daniel K Hartline
Feb 19, 2021·Cell and Tissue Research·Gabriela HollmannSilvana Allodi

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