PMID: 2501757Jun 26, 1989Paper

Regions at the carboxyl end of bacteriophage phi 29 protein p6 required for DNA binding and activity in phi 29 DNA replication

Nucleic Acids Research
M J Otero, Margarita Salas

Abstract

Series of deletions corresponding to the carboxyl end of the phage phi 29 protein p6 have been constructed and their activity in the initiation of phi 29 DNA replication and their capacity to interact with the phi 29 DNA ends have been studied. Determination of the activity of the deletion mutants in phi 29 DNA replication indicated the dispensability of the 14 carboxy-terminal amino acids of the protein. The activity of protein p6 decreased with deletions from 23 to 39 amino acids and was undetectable when 44 amino acids were removed. A similar behaviour was obtained when the interaction of the mutant proteins with the phi 29 DNA ends was analyzed. These results indicate that the stimulation of phi 29 DNA replication by protein p6 requires a specific binding to the phi 29 DNA ends.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1976·European Journal of Biochemistry·J L CarrascosaM Salas
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·I PrietoJ M Hermoso
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L Blanco, M Salas
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L Blanco, M Salas
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K WatabeJ Ito
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J M Hermoso, M Salas

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Citations

Aug 1, 1995·FEMS Microbiology Reviews·M SalasL Blanco
Jan 1, 1994·Biochimie·M SerranoJ M Hermoso
Sep 25, 1999·Journal of Molecular Biology·A M AbrilJ M Hermoso
Apr 6, 2006·FEMS Microbiology Reviews·Christoph Weigel, Harald Seitz
May 25, 1990·Science·M SerranoJ M Hermoso

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