Regions of high antigenicity within the hypothetical PPE major polymorphic tandem repeat open-reading frame, Rv2608, show a differential humoral response and a low T cell response in various categories of patients with tuberculosis

The Journal of Infectious Diseases
Prachee ChakhaiyarSeyed E Hasnain

Abstract

The function of the PE/PPE families of proteins, which represent approximately 10% of the coding capacity of the Mycobacterium tuberculosis genome, has remained relatively unknown. We earlier described a PPE family member, Rv2430c, as an immunodominant antigen. We now report another PPE family gene, Rv2608, a member of the major polymorphic tandem repeat subfamily, for its ability to elicit a high humoral and a low T cell response. Rv2608 was also found to be polymorphic in different clinical isolates of M. tuberculosis, as determined by polymerase chain reaction-restriction fragment-length polymorphism analysis. A total of 51 clinically confirmed patients with tuberculosis (TB), belonging to 3 different categories--fresh infection (n=22), relapsed infection (n=21), and extrapulmonary infection (n=8)--and 10 healthy control subjects were included in the study. Recombinant Rv2608 protein showed positive reactivity to patients' serum samples. Enzyme-linked immunosorbent assays and T cell-proliferation assays with synthetic peptides corresponding to predicted regions of high antigenicity showed a predominantly humoral response in patients with relapsed TB. We additionally identified the Gly-X-Gly-Asn-X-Gly repeat motifs as being p...Continue Reading

Citations

Jun 4, 2013·Marine Biotechnology·Satoru KurokawaTakashi Aoki
Apr 9, 2008·Nature Reviews. Microbiology·Niyaz AhmedSeyed E Hasnain
Dec 18, 2010·Astrobiology·Gene D McDonald, Michael C Storrie-Lombardi
Aug 20, 2010·Clinical and Vaccine Immunology : CVI·Gregory C IretonSteven G Reed
Mar 27, 2007·Journal of Bacteriology·Sharmistha BanerjeeSeyed E Hasnain
Feb 15, 2011·Clinical & Developmental Immunology·Samantha L Sampson
Sep 23, 2009·BMC Evolutionary Biology·Christopher R E McEvoyNicolaas C Gey van Pittius
Jun 25, 2014·Future Microbiology·Sam Willcocks, Brendan W Wren
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Guglielmo LuccheseDarja Kanduc
Apr 30, 2011·Tuberculosis·Sangita Mukhopadhyay, Kithiganahalli Narayanaswamy Balaji
Mar 19, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Sharmistha BanerjeeSeyed E Hasnain
Mar 7, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Andreas DemuthJörg Hacker
Jun 29, 2005·Trends in Microbiology·Jeffrey P Murry, Eric J Rubin
Sep 15, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Philip Raj AbrahamSangita Mukhopadhyay
Jul 6, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Philip Raj AbrahamSangita Mukhopadhyay
Aug 31, 2007·FEMS Immunology and Medical Microbiology·Laura RindiCarlo Garzelli
Mar 3, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·H ZhangH Wang
Jan 31, 2012·Journal of Molecular Microbiology and Biotechnology·Krishnaveni MohareerSeyed E Hasnain

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