PMID: 9544701Apr 17, 1998Paper

Regions of the Escherichia coli primary sigma factor sigma70 that are involved in interaction with RNA polymerase core enzyme

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
H Nagai, N Shimamoto

Abstract

The sigma factors of bacterial RNA polymerase are required for recognition of promoters in transcription initiation. Most sigma factors share several regions with significant homology in their amino acid sequences (regions 1-4). Some primary sigma factors carry a large nonconserved segment between regions 1 and 2. The binding of an sigma factor to the core enzyme alters the structure and properties of the sigma factor, but little is known about the binding mechanism and subsequent reactions. In this report, we employed the protein footprinting method to investigate the alteration of the structure and function of Escherichia coli sigma70 by binding to core enzyme and promoter DNA. A segment between regions 1.1 and 1.2, and that in region 3.2, were preferentially cleaved by hydroxyl radicals. Upon binding to the core enzyme, segments in regions 1.1, 2, 3 and 4 were substantially protected, while cleavage at a small segment in region 4.2 was weakly enhanced. In a binary complex of holoenzyme and promoter DNA, additional segments in regions 2.4 and 4.2 were protected, while the protection at region 1.1 disappeared. The nonconserved acidic region of sigma70 in the holoenzyme became hypersensitive upon binding to DNA. These results s...Continue Reading

Citations

Apr 3, 2001·Current Opinion in Microbiology·R R Burgess, L Anthony
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·C A GrossB Young
Jul 20, 2010·Biochemistry. Biokhimii︠a︡·Yu A KhodakV L Drutsa
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Caroline A DavisRuth M Saecker
Jun 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Julio A CamareroTom W Muir
Jun 10, 2015·Journal of Molecular Biology·Emily F RuffM Thomas Record
Dec 9, 2008·Molecular Cell·Michael L GleghornKatsuhiko S Murakami
Apr 5, 2002·Molecular Cell·Elizabeth A CampbellSeth A Darst
Oct 7, 2003·Annual Review of Microbiology·Tanja M Gruber, Carol A Gross
Jul 29, 2016·Frontiers in Microbiology·Orlando SantillánGuillermo Dávila
Mar 29, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Frédéric CollandAnnie Kolb
Feb 21, 2002·The Journal of Biological Chemistry·Motoki SusaNobuo Shimamoto
Feb 7, 2001·The Journal of Biological Chemistry·R SenN Shimamoto
Apr 23, 2008·Current Protocols in Protein Science·Nick Loizos

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