PMID: 2495264Apr 1, 1989Paper

Regions of toxin A involved in toxin A excretion in Pseudomonas aeruginosa

Journal of Bacteriology
Abdul N HamoodB H Iglewski

Abstract

Toxin A is excreted by Pseudomonas aeruginosa as a mature 66,583-dalton protein. In this study, we used molecular cloning and deletion analysis to define specific regions of the toxin molecule involved in its excretion. Subclones that express either the amino terminus, the carboxy terminus, or toxin A molecules with internal deletions were constructed. The hypotoxigenic mutant PAO-T1 was used as a host for the expression of the toxin constructs. When overexpressed (by the presence of extra copies of the toxin A-positive regulatory gene, regA, in trans), toxin A-cross-reactive materials produced by most of these constructs were detected in the supernatant of PAO-T1. The supernatant of P. aeruginosa PAO-T1 contained proteolytic activity that degraded toxin A-derived products but not the intact toxin molecule. A single SalI intragenic deletion (coding for the leader peptide, the first 30 amino acids, and the last 305 amino acids of the toxin) resulted in a relatively stable product in the supernatant of PAO-T1. The product of the carboxy terminus construct (which codes for the last 305 amino acids of the toxin) was detected in the lysate of PAO-T1 only. The data suggest that the amino terminus region of toxin A (the leader peptide...Continue Reading

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B H Iglewski, D Kabat
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1977·Infection and Immunity·M L VasilB H Iglewski
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K BackmanW Gilbert
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R K TaylorJ J Mekalanos
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C Manoil, J Beckwith
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V S AlluredD B McKay
Jan 1, 1987·Antibiotics and Chemotherapy·M S HindahlB H Iglewski
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·V K ChaudharyI Pastan
Jan 1, 1985·Annual Review of Microbiology·D Oliver
Apr 1, 1985·Infection and Immunity·K Richardson, C D Parker
Nov 1, 1974·The Journal of Infectious Diseases·P V Liu
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G L GrayH L Heyneker
Oct 1, 1980·The Journal of Infectious Diseases·D E OhmanB H Iglewski
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski

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Citations

Apr 1, 1990·Molecular Microbiology·M J WickB H Iglewski
Sep 1, 1993·Molecular Microbiology·I PoquetA P Pugsley
Dec 15, 1995·Molecular & General Genetics : MGG·C S McVay, A N Hamood
Feb 1, 1990·Biochimie·A LazdunskiM Murgier
Mar 1, 1993·Microbiological Reviews·A P Pugsley
Jul 19, 2001·Genes & Development·V T Lee, O Schneewind

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