PMID: 9427515Jan 14, 1998Paper

Regions outside of the Bcl-2 homology domains, BH1 and BH2 protect a dopaminergic neuronal cell line from staurosporine-induced cell death

Brain Research. Molecular Brain Research
Y J OhK L O'Malley

Abstract

Recent evidence demonstrates that the proto-oncogene product, Bcl-2 can protect cells from a variety of cell death-inducing stimuli. Because previous studies have demonstrated that protein kinase (PK) pathways may be involved in the regulation of cell death, we tested various PK inhibitors for their effects on cell death in a dopaminergic neuronal cell line, MN9D, as well as the potential of Bcl-2 family members and structural mutants to block this process. Cells expressing either human Bcl-2 (MN9D/Bcl-2), or neomycin (MN9D/Neo; control cells) were treated with either staurosporine (0.25-2 microM) or trifluoperazine (10-100 microM). In control MN9D/Neo cells, both reagents led to a dose-dependent cell death with morphological features of apoptosis. Overexpression of Bcl-2 rescued cells from staurosporine-induced but not trifluoperazine-induced apoptotic cell death. Cell death induced by the specific PKC inhibitor, calphostin C was also significantly attenuated in MN9D/Bcl-2 cells indicating that a PKC pathway represents one mechanism by which Bcl-2 prevents staurosporine-induced cell death. Similarly, the Bcl-2 family member, Bcl-X(L) also blocked staurosporine-induced cell death in MN9D cells whereas overexpression of Bcl-X(S)...Continue Reading

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Citations

Aug 24, 1999·Neurochemistry International·N Marks, M J Berg
Sep 19, 2003·Neurobiology of Disease·Karen L O'MalleyWilliam Holtz
Apr 21, 2016·Indian Journal of Nuclear Medicine : IJNM : the Official Journal of the Society of Nuclear Medicine, India·Ümmühan AbdülrezzakÖzgül Turhal
Feb 14, 2003·Journal of Neuroscience Research·Yan LongKeyi Yang
Aug 12, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jia YuBaixiang Li
Mar 20, 2003·The Journal of Biological Chemistry·Makoto Kawatani, Masaya Imoto

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