Regioselective carboxylation of catechol by 3,4-dihydroxybenzoate decarboxylase of Enterobacter cloacae P

Biotechnology Letters
T YoshidaT Nagasawa

Abstract

3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-free extract, catechol at 50 mM was carboxylated in the presence of 3 M KHCO(3) to 3,4-dihydroxybenzoic acid with a molar conversion ratio of 28% after 14 h at 30 degrees C.

References

Jan 1, 2003·Bioscience, Biotechnology, and Biochemistry·Toyokazu YoshidaToru Nagasawa
May 10, 2006·Applied Microbiology and Biotechnology·Tsuyoshi MatsuiToru Nagasawa

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Citations

Jul 25, 2013·Journal of Biotechnology·Christiane WuenschKurt Faber
Nov 21, 2012·Metabolic Engineering·Kathleen A CurranHal S Alper
Oct 30, 2010·Current Opinion in Biotechnology·Roland Wohlgemuth
Apr 30, 2017·Advanced Synthesis & Catalysis·Katharina PlaschSilvia M Glueck
Jan 7, 2016·Beilstein Journal of Organic Chemistry·Apostolos Alissandratos, Christopher J Easton
Aug 6, 2019·Advanced Synthesis & Catalysis·Stefan E PayerSilvia M Glueck
Apr 10, 2019·Applied Microbiology and Biotechnology·Daniel P BrinkMarie F Gorwa-Grauslund
Mar 21, 2021·Applied and Environmental Microbiology·Joydeep ChakrabortyHideaki Nojiri
Apr 5, 2012·Organic Letters·Christiane WuenschKurt Faber

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