Regioselective sulfation of Artemisia sphaerocephala polysaccharide: Characterization of chemical structure

Carbohydrate Polymers
Junlong WangZiqiang Lei

Abstract

The biological activities of sulfated polysaccharides are related to the substitution positions of functional groups. In this study, regioselective sulfation of Artemisia sphaerocephala polysaccharides (SRSASP) was prepared by using triphenylchloromethane (TrCl) as protecting precursor. FT-IR spectra and X-ray photoelectron spectroscopy (XPS) showed that SO(3-) group (S(6+), high binding energy of 168.7eV) was widely present in sulfated polysaccharides. (13)C NMR spectroscopy showed that C-2 and C-3 substitution was occurred but not fully sulfation. Meanwhile, C-6 substituted signals near 65ppm were not observed. The degree of substitution varied from 0.44 to 0.63 in SRSASP which could be attributed to the low reactivity at secondary hydroxyl. Monosaccharide composition result showed a decrease in the ratio of mannose/glucose, indicating the change of chemical composition in sulfated polysaccharides. In size-exclusion chromatograph analysis, a decrease in molecular weight and broadening of molecular weight distribution of sulfated polysaccharides was also observed. It could be attributed to the hydrolysis of polysaccharide in the sulfated reaction.

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Oct 15, 2013·International Journal of Biological Macromolecules·Junlong WangYuting Zhao
Dec 17, 2014·International Journal of Biological Macromolecules·Junlong WangZiqiang Lei

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Citations

Feb 20, 2016·International Journal of Biological Macromolecules·Yunfei XuJi Zhang
Nov 5, 2016·International Journal of Biological Macromolecules·Junlong WangZiqiang Lei
Dec 17, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yun ChenJiaguo Liu
Jan 29, 2021·International Journal of Biological Macromolecules·Zeineb Mzoughi, Hatem Majdoub
Jun 6, 2021·International Journal of Biological Macromolecules·Shen SongZengjie Fan

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