Registered report: A coding-independent function of gene and pseudogene mRNAs regulates tumour biology

ELife
Dale CowleyReproducibility Project Cancer Biology

Abstract

The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (Errington et al., 2014). This Registered report describes the proposed replication plan of key experiments from 'A coding-independent function of gene and pseudogene mRNAs regulates tumour biology' by Poliseno et al. (2010), published in Nature in 2010. The key experiments to be replicated are reported in Figures 1D, 2F-H, and 4A. In these experiments, Poliseno and colleagues report microRNAs miR-19b and miR-20a transcriptionally suppress both PTEN and PTENP1 in prostate cancer cells (Figure 1D; Poliseno et al., 2010). Decreased expression of PTEN and/or PTENP1 resulted in downregulated PTEN protein levels (Figure 2H), downregulation of both mRNAs (Figure 2G), and increased tumor cell proliferation (Figure 2F; Poliseno et al., 2010). Furthermore, overexpression of the PTEN 3' UTR enhanced PTENP1 mRNA abundance limiting tumor cell proliferation, providing additional evidence...Continue Reading

References

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Citations

Dec 24, 2015·Frontiers in Medicine·Cristina Sorina CătanăIoana Berindan-Neagoe
Jan 30, 2013·PLoS Genetics·Daniel R SchriderSandro J de Souza
Nov 28, 2013·PloS One·Deniz KanberDietmar Lohmann
Sep 17, 2013·PloS One·Emőke-Ágnes HorvátKatharina Anna Zweig
Sep 28, 2013·PloS One·Izabella Slezak-ProchazkaAnke van den Berg
Jun 26, 2012·Cell·Shanker Kalyana-SundaramArul M Chinnaiyan
Apr 22, 2020·ELife·George A Calin
Apr 22, 2020·ELife·John KerwinUNKNOWN Reproducibility Project: Cancer Biology
Dec 8, 2021·ELife·Timothy M ErringtonBrian A Nosek

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Methods Mentioned

BETA
PCR
transfection
protein determination

Software Mentioned

R
R Development Core Team
Power

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