Registered report: Diverse somatic mutation patterns and pathway alterations in human cancers

ELife
Vidhu SharmaReproducibility Project Cancer Biology

Abstract

The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (Errington et al., 2014). This Registered Report describes the proposed replication plan of key experiments from "Diverse somatic mutation patterns and pathway alterations in human cancers" by Kan and colleagues published in Nature in 2010 (Kan et al., 2010). The experiments to be replicated are those reported in Figures 3D-F and 4C-F. Kan and colleagues utilized mismatch repair detection (MRD) technology to identify somatic mutations in primary human tumor samples and identified a previously uncharacterized arginine 243 to histidine (R243H) mutation in the G-protein α subunit GNAO1 in breast carcinoma tissue. In Figures 3D-F, Kan and colleagues demonstrated that stable expression of mutant GNAO1(R243D) conferred a significant growth advantage in human mammary epithelial cells, confirming the oncogenic potential of this mutation. Similarly, expression of variants with somati...Continue Reading

References

Aug 10, 1990·Science·J LyonsH R Bourne
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Mar 10, 2001·Nature·L Chang, M Karin
Dec 26, 2001·Human Mutation·Gloria H SuScott E Kern
Apr 16, 2003·Nature·Francis S CollinsUNKNOWN US National Human Genome Research Institute
Aug 12, 2005·Nature·D Williams ParsonsVictor E Velculescu
Sep 6, 2005·Cancer Research·Helen DaviesP Andrew Futreal
Apr 22, 2006·Cancer Biology & Therapy·Kentaro NakayamaTian-Li Wang
Mar 9, 2007·Nature·Christopher GreenmanMichael R Stratton
Nov 6, 2007·Nature·Barbara A WeirMatthew Meyerson
Apr 23, 2008·Current Protocols in Human Genetics·S A ForbesM R Stratton
Dec 17, 2008·Nature·Catherine D Van RaamsdonkBoris C Bastian
Apr 11, 2009·Nature·Michael R StrattonP Andrew Futreal
Jul 18, 2009·International Journal of Cancer. Journal International Du Cancer·Yisheng WeiJianping Wang
Dec 18, 2009·Nature·Erin D PleasancePeter J Campbell
Mar 24, 2010·International Journal of Cancer. Journal International Du Cancer·Shamima YeasminKohji Miyazaki
Jul 9, 2010·Cancer Research·Katherine G Finegan, Cathy Tournier
Jul 30, 2010·Nature·Zhengyan KanSomasekar Seshagiri
Aug 17, 2010·Genome Medicine·Gerd P Pfeifer
Feb 15, 2011·Oncogene·M Garcia-MarcosM G Farquhar
Mar 17, 2011·Genes & Development·Lynda ChinMatthew Meyerson
Apr 14, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shamima YeasminKohji Miyazaki
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Sep 12, 2013·Nature Reviews. Genetics·Ian R WatsonLynda Chin
Sep 21, 2013·Amino Acids·Beata Jastrzebska
Feb 4, 2014·Biochimica Et Biophysica Acta·Wiebke HaeusgenVicki Waetzig
Jul 2, 2014·Nature Reviews. Genetics·Thomas HelledaySerena Nik-Zainal
Jul 2, 2014·The Journal of Biological Chemistry·Anthony LeymeMikel Garcia-Marcos
Dec 11, 2014·ELife·Timothy M ErringtonBrian A Nosek

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Citations

Jan 23, 2018·Omics : a Journal of Integrative Biology·Kevin DzoboM Iqbal Parker
Dec 8, 2021·ELife·Timothy M ErringtonBrian A Nosek

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Methods Mentioned

BETA
PCR
transfection
FACS
flow cytometry
X-ray
Flow
Assay
Protein determination assay

Software Mentioned

Power
GelCount
ImageJ
R

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