Registration of T-2 mycotoxin with total internal reflection ellipsometry and QCM impedance methods

Biosensors & Bioelectronics
Alexy V NabokO Gojster

Abstract

A sensitive optical method of total internal reflection ellipsometry (TIRE) in conjunction with immune assay approach was exploited for the registration of T-2 mycotoxin in a wide range of concentrations from 100 microg/ml down to 0.15 ng/ml. Association constants of 1.4x10(6) and 1.9x10(7)mol(-1)s for poly- and monoclonal T-2 antibodies, respectively, were evaluated from TIRE kinetic measurements. According to TIRE data fitting, binding of T-2 molecules to antibodies (at saturation) has resulted in the increase in adsorbed layer thickness of 4-5 nm. The QCM impedance measurements data showed anomalously large mass increase and film softening, most likely, due to the binding of large T-2 aggregates to antibodies.

References

Jun 5, 2004·Applied Optics·Hans ArwinKnut Johansen
Feb 1, 2005·Biosensors & Bioelectronics·Dhesingh Ravi ShankaranNorio Miura

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Citations

Mar 5, 2013·ACS Applied Materials & Interfaces·Tianyu XueWeitao Zheng
Mar 31, 2009·International Journal of Molecular Sciences·Pietro CozziniChiara Dall'Asta
Jul 1, 2010·Prikladnaia biokhimiia i mikrobiologiia·A E UrusovB B Dzantiev
Sep 4, 2012·Biosensors & Bioelectronics·Ieva BaleviciuteArunas Ramanavicius
Feb 19, 2016·Analytical Chemistry·Wei LiuGang Jin
May 12, 2009·Seminars in Cell & Developmental Biology·Paul J ConroyRichard J O'Kennedy
Jul 20, 2011·Biophysical Journal·Verena KriechbaumerAlexei Nabok
Jan 12, 2012·The Analyst·Mònica CampàsBeatriz Prieto-Simón
Aug 4, 2011·Journal of Molecular Recognition : JMR·Bernd Becker, Matthew A Cooper
Jul 28, 2020·Critical Reviews in Analytical Chemistry·Mustafa Oguzhan CaglayanZafer Üstündağ
Jul 10, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Alexei NabokAnna Demchenko
Jun 16, 2007·Biosensors & Bioelectronics·Alexei NabokSéamus P J Higson

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