PMID: 9450663Feb 5, 1998Paper

Regucalcin increases Ca2+-ATPase activity and ATP-dependent calcium uptake in the microsomes of rat kidney cortex

Molecular and Cellular Biochemistry
H Kurota, M Yamaguchi

Abstract

The effect of regucalcin, a Ca2+-binding protein, on Ca2+ transport system in rat renal cortex microsomes was investigated. The presence of regucalcin (10[-8] to 10[-6] M) in the reaction mixture caused a significant increase in Ca2+-ATPase activity and ATP-dependent 45Ca2+ uptake in the microsomes. Regucalcin (10[-7] M) increased Ca2+-ATPase activity independently of increasing concentrations of CaCl2. The microsomal Ca2+-ATPase activity and 45Ca2+ uptake were markedly decreased by the presence of vanadate (0.1 mM) or N-ethylmaleimide (NEM; 5 mM) in the absence or presence of regucalcin. Dithiothreitol (DTT; 5 mM) markedly elevated Ca2+-ATPase activity and 45Ca2+ uptake in the microsomes. The DTT effects were not further enhanced by regucalcin (10[-7] M). Meanwhile, the microsomal Ca2+-ATPase activity and 45Ca2+ uptake were significantly decreased by the presence of dibutyryl cyclic AMP (DcAMP; 10[-5] and 10[-3] M) or inositol 1,4, 5-trisphosphate (IP3; 10[-7] and 10[-5] M). The effect of regucalcin (10[-7] M) on Ca2+ATPase activity and 45Ca2+ uptake was weakened in the presence of DcAMP or IP3. The present results demonstrate that regucalcin has a stimulatory effect on ATP-dependent Ca2+ uptake in the microsomes of rat renal ...Continue Reading

Citations

Sep 24, 2015·International Journal of Molecular Medicine·Masayoshi Yamaguchi
Mar 24, 2005·Journal of Cellular Biochemistry·Taeko Nakagawa, Masayoshi Yamaguchi
Mar 25, 2011·Molecular and Cellular Biochemistry·Masayoshi Yamaguchi
Nov 29, 2011·Molecular Human Reproduction·Sandra S LaurentinoSílvia Socorro
Aug 14, 2001·Journal of Cellular Biochemistry·Y Morooka, M Yamaguchi
Jul 12, 2002·Journal of Cellular Biochemistry·Masayoshi Yamaguchi, Rie Nakajima
Sep 28, 2000·Biochemical and Biophysical Research Communications·M Yamaguchi
May 16, 2000·Life Sciences·M Yamaguchi

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