Regucalcin increases Ca2+-ATPase activity in the mitochondria of brain tissues of normal and transgenic rats

Journal of Cellular Biochemistry
Masayoshi YamaguchiTaeko Nakagawa

Abstract

The role of regucalcin, which is a regulatory protein in intracellular signaling, in the regulation of Ca(2+)-ATPase activity in the mitochondria of brain tissues was investigated. The addition of regucalcin (10(-10) to 10(-8) M), which is a physiologic concentration in rat brain tissues, into the enzyme reaction mixture containing 25 microM calcium chloride caused a significant increase in Ca(2+)-ATPase activity, while it did not significantly change in Mg(2+)-ATPase activity. The effect of regucalcin (10(-9) M) in increasing mitochondrial Ca(2+)-ATPase activity was completely inhibited in the presence of ruthenium red (10(-7) M) or lanthanum chloride (10(-7) M), both of which are inhibitors of mitochondrial uniporter activity. Whether the effect of regucalcin is modulated in the presence of calmodulin or dibutyryl cyclic AMP (DcAMP) was examined. The effect of regucalcin (10(-9) M) in increasing Ca(2+)-ATPase activity was not significantly enhanced in the presence of calmodulin (2.5 microg/ml) which significantly increased the enzyme activity. DcAMP (10(-6) to 10(-4) M) did not have a significant effect on Ca(2+)-ATPase activity. The effect of regucalcin (10(-9) M) in increasing Ca(2+)-ATPase activity was not seen in the pres...Continue Reading

References

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Citations

Mar 23, 2013·Cellular and Molecular Life Sciences : CMLS·Ricardo MarquesSílvia Socorro
Jun 2, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Masayoshi Yamaguchi
Mar 25, 2011·Molecular and Cellular Biochemistry·Masayoshi Yamaguchi

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