PMID: 15375603Sep 18, 2004Paper

Regucalcin increases superoxide dismutase activity in the heart cytosol of normal and regucalcin transgenic rats

International Journal of Molecular Medicine
Emiko Ichikawa, M Yamaguchi

Abstract

The effect of regucalcin, a regulatory protein in the intracellular signaling system, on superoxide dismutase (SOD) activity in the heart cytosol of normal rats and regucalcin transgenic (TG) rats was investigated. The addition of regucalcin (10(-10) to 10(-8) M) with a physiologic concentration in the enzyme reaction mixture containing the heart cytosol obtained from normal rats caused a significant increase in SOD activity, indicating that regucalcin directly activates the enzyme. The effect of regucalcin (10(-8) M) in increasing SOD activity was not seen in the presence of dithiothreitol (DTT; 0.1 or 1.0 mM), a protecting reagent for sulfhydryl group, or N-ethylmaleimide (NEM; 0.1 or 1.0 mM), a modifying reagent for sulfhydryl group, in the reaction mixture, indicating that regucalcin does not affect the sulfhydryl group. The addition of zinc sulfate (10(-6) to 10(-4) M) in the reaction mixture did not cause a significant change in SOD activity, while the enzyme activity was markedly decreased in the presence of cupric sulfate (10(-6) to 10(-4) M). The activatory effect of regucalcin (10(-8) M) on SOD was seen in the presence of zinc (10(-4) M), while not observed in the presence of copper (10(-4) M). Moreover, SOD activity ...Continue Reading

Citations

Mar 23, 2013·Cellular and Molecular Life Sciences : CMLS·Ricardo MarquesSílvia Socorro
Aug 26, 2006·The European Journal of Neuroscience·Liwen SunYinghe Hu
Apr 26, 2016·Journal of Applied Toxicology : JAT·Sara CorreiaSílvia Socorro

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