PMID: 8944661Nov 1, 1996Paper

Regulated calcium channel in apical membranes renal proximal tubule cells

The American Journal of Physiology
M I Zhang, R G O'Neil

Abstract

Using the single-channel patch-clamp technique, we identified a Ca2+ channel in the apical membranes rabbit cultured proximal tubule cells. The channel is permeable to both Ca2+ and Ba2+ but not to monovalent cations. In on-cell patches, the channel opened infrequently and had a conductance of 4.6 +/- 0.9 pS (n = 5) with 105 mM CaCl2 in the pipette. Although addition of forskolin (12.5 microM) Cl2 is without effect, addition of phorbol 12-myristate 13-acetate (PMA, 1 microM) activated the channel. At 0 mV pipette voltage (resting state) in the on-cell patches, PMA increased open probability (P0) from 0 to 6.9 +/- 2.3% (n = 5) within 1-3 min of PMA application. Likewise, stretching the membrane patch (-10 to -30 mmHg) activated this channel (P0 increased to 5.3 +/- 2.1%, n = 3, at 0 mV applied pipette potential), with results consistent with a mechanosensitive channel. The channel displayed only modest voltage sensitivity, with mild activation on membrane hyperpolarization but with inactivation on strong depolarization. The addition of the L-type Ca2+ channel blocker (antagonist), nifedipine (10 microM), completely blocked this channel in both on-cell and inside-out patches, whereas the agonist, BAY K 8644 (5 microM) was without...Continue Reading

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Citations

Nov 14, 2003·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Roger G O'Neil, Rachel C Brown
Mar 25, 2016·American Journal of Physiology. Renal Physiology·Matthias B Moor, Olivier Bonny
May 2, 2019·American Journal of Physiology. Renal Physiology·Shane A WiebeR Todd Alexander
Jan 20, 1998·Drug and Chemical Toxicology·G L DiamondW L Ruoff
Jul 5, 2003·Physiological Reviews·Julian T Dow, Shireen A Davies
Mar 31, 2004·Canadian Journal of Physiology and Pharmacology·M G BrunetteY Bourgeois
May 31, 2018·American Journal of Physiology. Renal Physiology·Aurélie Edwards, Olivier Bonny
May 23, 1998·Drug and Chemical Toxicology·G L DiamondW L Ruoff
May 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·W WangS Nielsen
Dec 15, 2006·Journal of Cellular Physiology·Ji Yeon HanHo Jae Han
Jan 19, 2006·American Journal of Physiology. Renal Physiology·Yun Jung Lee, Ho Jae Han
Aug 8, 1997·Biochemical and Biophysical Research Communications·R G O'NeilN J Karin
May 9, 2000·Biochemical and Biophysical Research Communications·J M Reid, R G O'Neil
Feb 13, 2001·Biochemical and Biophysical Research Communications·M I Zhang, R G O'Neil
Mar 21, 1998·Kidney International·S M PetersR J Bindels
Mar 13, 2001·Cell Calcium·M I Zhang, R G O'Neil

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