Regulated exocytosis and sequential construction of the extracellular matrix surrounding the sea urchin zygote

Developmental Biology
J C MateseD R McClay

Abstract

After fertilization most eggs become surrounded by a complex extracellular matrix. This study examines those matrix assembly processes that are triggered by fertilization of the sea urchin egg. The study uses antibodies that identify five different storage compartments in the egg. These compartments release their protein contents in a highly regulated fashion to assemble and modify the extraembryonic layers. The exocytosis sequence begins with a fertilization wave that progresses from the site of sperm entry and elevates the fertilization envelope above a water-filled perivitelline space. The immediate surface of the zygote then becomes covered by a newly secreted hyaline layer. Prior to fertilization some of the antigens are localized to cortical granules. Others are found in "basal laminar vesicles" that are released in a wave beginning at about 30 sec, or roughly at the same time as cortical granule exocytosis. The remaining antigens are exocytosed with a rather precise timing, but with a delay of several to tens of minutes relative to the first wave of exocytosis. "Apical vesicles," so named because antigens from this class are preferentially exocytosed toward the apical cell surface of polarized cells, include antigens tha...Continue Reading

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Citations

Jan 6, 2001·Molecular Reproduction and Development·S D Conner, G M Wessel
Jul 4, 1998·Zygote : the Biology of Gametes and Early Embryos·J C Matese, D R McClay
Jan 10, 2001·Development, Growth & Differentiation·M Masui, T Kominami
Aug 15, 2002·Development, Growth & Differentiation·Janice Mayne, John J Robinson
Jun 23, 2004·Development, Growth & Differentiation·Koichi H KatoYukio Yokota
Apr 10, 2007·Eukaryotic Cell·Talibah MetcalfChristopher M West
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Min LiuPrue Talbot
Nov 18, 2011·Reproductive Biology and Endocrinology : RB&E·Min Liu
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Britten
Jan 22, 2011·Cell Calcium·Anthony J Morgan
Jan 11, 2013·Development, Growth & Differentiation·Daniele P RomancinoMaria Di Bernardo
Jan 11, 2002·Developmental Biology·Christian SardetJanet Chênevert
Oct 7, 2004·Gene Expression Patterns : GEP·Mariana Leguia, Gary M Wessel
Mar 28, 2006·Current Topics in Developmental Biology·Julian L Wong, Gary M Wessel
Jan 27, 1998·The Journal of Biological Chemistry·V V ChestkovS S Vogel
Oct 26, 2007·The Journal of Biological Chemistry·Anthony J Morgan, Antony Galione

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