PMID: 8604014Mar 1, 1996Paper

Regulated expression of a 16-kd galectin-like protein in activated rat macrophages

Journal of Leukocyte Biology
G A RabinovichC E Sotomayor

Abstract

We investigated the presence of a galectin-like protein in rat mononuclear cells using a polyclonal antibody raised against a soluble lactose-binding lectin purified from adult chicken liver that immunoreacted strongly with a broad protein band of about 16 kd in Western blot assays. Immunochemical studies revealed a constitutive expression of this protein in mononuclear cells mainly in the macrophage (M phi) population. Subcellular localization was assessed by Western blot assays of the cytosolic and membrane fractions of different cell populations studied: (1) spleen mononuclear cells, (2) T cell-enriched, (3) B cell- and M phi-enriched populations, and (4) peritoneal cells, processed in the presence of lactose. In broad agreement with immunocytochemical studies of nonpermeabilized and permeabilized cells, Western blot assays suggest that this protein is localized mainly in the cytoplasmic compartment but also associated with the cell surface. By flow cytometric analyses we detected about a 14% of ED1 double-positive cells corresponding to macrophages that constitutively express this galectin-like protein associated with their cell surface. The cytosolic fraction obtained from the M phi-enriched cell population showed hemagglu...Continue Reading

Citations

Jan 31, 2003·The Journal of Biological Chemistry·Jacob RachmilewitzMark L Tykocinski
Apr 25, 2012·Annals of the New York Academy of Sciences·Sachiko SatoMichel J Tremblay
Feb 15, 2013·American Journal of Reproductive Immunology : AJRI·Ada G Blidner, Gabriel A Rabinovich
Sep 10, 2015·FEBS Letters·Ada G BlidnerGabriel A Rabinovich
Jul 19, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roberto C DavicinoGabriel A Rabinovich
Dec 14, 2019·Hematological Oncology·Johanne Marie HolstMartin Bjerregård Pedersen
Apr 9, 2020·Frontiers in Pharmacology·Hannah L LawDianne Cooper
Nov 27, 1999·The Journal of Biological Chemistry·H PyoI Jou
Dec 24, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Atsushi MasamuneTooru Shimosegawa
Nov 22, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Victoria SundbladGabriel A Rabinovich
Nov 13, 2018·Frontiers in Immunology·Urban Švajger, Primož Rožman
Mar 6, 2019·Mini Reviews in Medicinal Chemistry·Narella Sridhar GoudMallika Alvala
Jan 6, 2001·Memórias do Instituto Oswaldo Cruz·G A Rabinovich
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sebastián M MallerKarina V Mariño
Jul 21, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G A RabinovichC E Sotomayor
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G A RabinovichC E Sotomayor
Aug 3, 1999·The Journal of Experimental Medicine·G A RabinovichY Chernajovsky
Jun 20, 2014·FEBS Letters·Cristina Escoda-FerranFrancisco Lozano
Dec 14, 2018·Mediators of Inflammation·Ignacio M SeropianGabriel A Rabinovich
Nov 9, 2011·Antimicrobial Agents and Chemotherapy·Christian St-PierreMichel J Tremblay
Dec 25, 2010·Journal of Clinical Immunology·Juan P CerlianiGabriel A Rabinovich
Jun 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Agustin L LujanGabriel A Rabinovich
Jan 27, 2021·Glycobiology·Anabela M CutineGabriel A Rabinovich
Nov 19, 2020·Molecular Aspects of Medicine·Lei WanFu-Tong Liu
May 21, 2011·Toxicology Letters·Yu-Ying ChangRong-Nan Huang

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