Regulated expression of endothelial lipase in atherosclerosis

Molecular and Cellular Endocrinology
Xiaoqian WuPeiqing Liu

Abstract

Endothelial lipase (EL) is a major determinant of HDL metabolism and associated with the development of atherosclerosis, however the regulated expression of EL in atherosclerosis is unclear. In this study, we investigated EL expression in rat atherosclerosis and explored the potential mechanisms regulating EL expression by employing LPS on Raw264.7 cells in vitro. Rat atherosclerosis model was established fed on high-cholesterol diet (HCD) combined with vitamin D(2) (VD). Western blotting and immunochemistry staining revealed that EL expression was increased in the aorta, especially the atherosclerotic lesions in HCD rats. LPS increased EL expression in a time and dose dependent manner in Raw264.7 cells and NFkappaB inhibitor, PDTC attenuated the effects of LPS on EL. EMSA revealed that LPS stimulated NFkappaB binding to EL promoter. In summary, EL was upregulated in rat atherosclerosis and LPS stimulates EL expression in vitro through NFkappaB activation.

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Citations

Aug 5, 2010·Current Opinion in Lipidology·Gunilla Olivecrona, Thomas Olivecrona
Oct 9, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Un Ju JungRichard J Deckelbaum
Oct 15, 2010·Journal of Lipid Research·Kenneth R Feingold, Carl Grunfeld
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Nov 13, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yi CaiPei-Qing Liu
Jun 9, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Suowen XuPeiqing Liu
Dec 6, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Kunming TianPeiqing Liu
Aug 9, 2016·Current Opinion in Lipidology·Kenneth R Feingold, Carl Grunfeld
Feb 8, 2019·Scientific Reports·Jinping WangZheng Gen Jin
Oct 28, 2017·Frontiers in Pharmacology·Jian Fang, Yongjun Zhang
Jan 10, 2019·Lipids in Health and Disease·Shuo YangQing-Hui Zhang
May 19, 2021·Scientific Reports·Manuela Gago-DominguezJ Esteban Castelao

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