Regulated expression of pathogen-associated molecular pattern molecules in Staphylococcus epidermidis: quorum-sensing determines pro-inflammatory capacity and production of phenol-soluble modulins

Cellular Microbiology
C VuongMichael Otto

Abstract

Phenol-soluble modulin (PSM) is a peptide complex produced by the nosocomial pathogen Staphylococcus epidermidis that has a strong capacity to activate the human innate immune response. We developed a novel method based on liquid chromatography-mass spectrometry (LC-MS) to quantify the production of the individual PSM components. Each PSM peptide was abundant in most of the 76 S epidermidis strains tested. Importantly, none of the PSM components were secreted by an agr mutant strain, indicating that PSM synthesis is regulated strictly by the agr quorum-sensing system. Furthermore, the agr mutant strain failed to elicit production of TNFalpha by human myeloid cells and induced significantly less neutrophil chemotaxis compared with the wild-type strain. Thus, quorum-sensing in S. epidermidis dramatically influenced activation of human host defence. We propose that an agr quorum-sensing mechanism facilitates growth and survival in infected hosts by adapting production of the pro-inflammatory PSMs to the stage of infection.

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Citations

Jul 30, 2010·Cellular and Molecular Life Sciences : CMLS·Natalia Malachowa, Frank R DeLeo
Sep 7, 2006·Analytical and Bioanalytical Chemistry·Dietrich MackJohannes K-M Knobloch
Nov 19, 2011·Seminars in Immunopathology·Michael Otto
Oct 2, 2013·Trends in Microbiology·Yaara Oppenheimer-ShaananIlana Kolodkin-Gal
Dec 23, 2010·Chemical Reviews·Matthew ThoendelAlexander R Horswill
Feb 12, 2013·Nature Medicine·Som S ChatterjeeMichael Otto
Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Saravanan PeriasamyMichael Otto
Dec 25, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maren RautenbergAndreas Peschel
Jul 16, 2005·Clinical Microbiology Reviews·Bénédicte Fournier, Dana J Philpott
Dec 21, 2010·Journal of Bacteriology·Eleni TsompanidouEmma L Denham
Jan 10, 2008·BMC Microbiology·Min LiMichael Otto
Oct 16, 2010·PLoS Pathogens·Gordon Y C CheungMichael Otto
Jun 5, 2010·Future Microbiology·Paul D Fey, Michael E Olson
Jan 23, 2014·International Journal of Medical Microbiology : IJMM·Michael Otto
Mar 29, 2013·Future Microbiology·Garry LavertyBrendan F Gilmore
Jun 25, 2014·Future Microbiology·Rachna Singh, Pallab Ray
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Sep 4, 2007·Biomaterials·Lucio MontanaroCarla Renata Arciola
Jun 22, 2007·International Journal of Medical Microbiology : IJMM·Karsten BeckerWilma Ziebuhr
Oct 31, 2015·Future Microbiology·Sharon KleinschmidtLouise M Hafner
Jul 15, 2009·Biotechnology and Applied Biochemistry·Sheetal RainaTajalli Keshavarz
Oct 13, 2011·IUBMB Life·Gustavo M Cerqueira, Anton Y Peleg
Feb 21, 2006·International Journal of Medical Microbiology : IJMM·Kok-Fai KongMichael Otto
Feb 5, 2011·Microbiology·Sarah E RoweJames P O'Gara
Jan 1, 2014·FEMS Microbiology Reviews·Gordon Y C CheungMichael Otto
Aug 17, 2012·Communicative & Integrative Biology·Saravanan PeriasamyMichael Otto
Feb 25, 2006·Cell·David M RaskinJohn J Mekalanos

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