PMID: 8974011Dec 1, 1996Paper

Regulated expression of the interferon-induced protein kinase p68 (PKR) by vaccinia virus recombinants inhibits the replication of vesicular stomatitis virus but not that of poliovirus

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
S B LeeM Esteban

Abstract

A direct antiviral role of the interferon-induced human protein kinase p68 has been shown only against encephalomyocarditis virus (EMCV) and vaccinia virus (VV). To determine if p68 kinase (PKR) has a broad antiviral effect, we have used coinfections between VV recombinants expressing p68 kinase under regulation of the lac I operator/repressor elements of Escherichia coli and two RNA viruses, vesicular stomatitis virus (VSV) and poliovirus. In cells coinfected with VV recombinants and VSV, induction with isopropyl-B-D-thiogalactoside (IPTG) of wild-type p68 kinase or a mutant lacking the dsRNA binding domain resulted in inhibition of both VV and VSV protein synthesis. This inhibition is not observed in cells infected with a catalytically inactive point mutant lys-arg296 of p68 kinase. When cells are coinfected with VV recombinants and poliovirus, induction of active p68 kinase resulted in a decrease in VV proteins but not in poliovirus proteins or poliovirus yields. Immunoblot analysis revealed that p68 kinase was expressed during mixed infections. Our results demonstrate a differential effect of p68 kinase on the replication of VV, VSV, and poliovirus. We suggest that in a particular virus-cell system, the different sensitivit...Continue Reading

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Citations

Jun 26, 2002·Viral Immunology·Martin Rottenberg, Krister Kristensson
May 3, 2003·Viral Immunology·David A CheslerCarol Shoshkes Reiss
Feb 3, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jesús Gil, Mariano Esteban
May 18, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Bobbie Ann AustinDaniel J J Carr
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bobbie Ann AustinDaniel J J Carr
May 15, 2007·Neuro-Signals·Raymond Chuen-Chung ChangCora Sau-Wan Lai
Nov 26, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·C RivasM Esteban
Aug 5, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·K S KhabarB R Williams
May 4, 2004·Molecular and Cellular Biology·Jesús GilMariano Esteban
May 29, 2003·The Journal of General Virology·Claudia González-LópezJavier Benavente
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·M A GarcíaM Esteban
Sep 1, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Martina Rieder, Karl-Klaus Conzelmann
Mar 7, 2021·International Journal of Molecular Sciences·Valentina MarchicaNicola Giuliani
Oct 1, 2003·Virology·Michael J StewartBarbara Sherry
Apr 25, 2007·Biochimie·M A GarcíaM Esteban

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