PMID: 6162853Feb 1, 1981Paper

Regulated transcription of the genes for actin and heat-shock proteins in cultured Drosophila cells

The Journal of Cell Biology
R C Findly, T Pederson

Abstract

The transcription of three specific genes has been examined in heat-shocked drosophila cells by hybridizing pulse-labeled nuclear RNA with cloned DNA sequences. Actin gene transcription is rapidly and profoundly suppressed upon heat shock but returns to near- normal levels after cells are placed back at their normal culture temperature (23 degrees C). Conversely, the transcription of genes coding from 70,000- and 26,000-dalton heat- shock proteins increases dramatically and with extraordinary rapidity (60 s) after heat shock. The temporal patterns of 70,000- and 26,000-dalton heat-shock gene transcription are nearly superimposable, indicating that, although they are closely linked cytologically, these genes are nevertheless tightly coregulated. The abundance of heat- shock gene transcripts reaches remarkable levels, e.g., 70,000-dalton heat-shock gene transcripts account for 2-3 percent of the nuclear RNA labeled during the first 30 min of heat shock. When heat-shocked cells are returned to 25 degrees C, the rates of transcription of the heat-shock genes fall back to the low levels characteristic of untreated cells. To confirm the low level of heat-shock gene transcription in normal cells, nuclear RNA was purified from unlabele...Continue Reading

References

Jun 1, 1979·Cell·M Ashburner, J J Bonner
Dec 20, 1979·Nucleic Acids Research·J P GergenP C Wensink
Aug 5, 1979·Journal of Molecular Biology·J O Carlson, D E Pettijohn
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M J MillerE P Geiduschek
Feb 5, 1977·Journal of Molecular Biology·A SpradlingS Penman
Nov 25, 1977·Journal of Molecular Biology·S L McKenzie, M Meselson
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S L McKenzieM Meselson
Apr 15, 1974·Journal of Molecular Biology·A TissièresU M Tracy
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·T Pederson
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Feb 1, 1980·Cell·E A FyrbergK L Kindle
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S C WadsworthB J McCarthy
Oct 1, 1980·Developmental Biology·W F Loomis, S Wheeler
Sep 1, 1980·Developmental Biology·D Francis, L Lin
Apr 25, 1980·Journal of Molecular Biology·C A HollandT Pederson

❮ Previous
Next ❯

Citations

Jan 1, 1991·Journal of Cellular Physiology·M M Sanders, C Kon
May 1, 1994·Molecular Biology Reports·J M Sierra, J M Zapata
Oct 1, 1983·Developmental Biology·E A CraigL J Manseau
Jan 1, 1993·Molecular Aspects of Medicine·R H Burdon
Jan 1, 1982·Bio Systems·L RensingK Drescher
Jun 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C R SantosG E Sweeney
Aug 10, 2004·Nature Structural & Molecular Biology·Celso A EspinozaJames A Goodrich
Aug 10, 2004·Nature Structural & Molecular Biology·Tiffany A AllenJennifer F Kugel
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·I V Economidis, T Pederson
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J W Cosgrove, I R Brown
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P P Di Nocera, I B Dawid
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A P McMahonE H Davidson
Nov 1, 1981·The Journal of Cell Biology·M M Sanders
Jan 1, 1983·The Journal of Cell Biology·J M VelazquezS Lindquist
Nov 1, 1983·The Journal of Cell Biology·T Pederson
Nov 1, 1983·The Journal of Cell Biology·J R SubjeckR J Johnson
Oct 1, 1984·The Journal of Cell Biology·R Morimoto, E Fodor
Oct 2, 2009·Molecular Biology of the Cell·Sabrina FritahClaire Vourc'h
Mar 1, 1984·European Journal of Biochemistry·L Nover, K D Scharf
Jan 15, 1988·European Journal of Biochemistry·A M CourgeonM Best-Belpomme
Jul 1, 1995·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·G C LiG Weitzel
Jan 1, 1982·CRC Critical Reviews in Biochemistry·I L CartwrightG C Howard
Jun 1, 1988·Journal of Cellular Physiology·S SadisL A Weber
Oct 1, 1987·Teratology·D A WalshM J Edwards
Jun 15, 1982·Biochemical and Biophysical Research Communications·B S Inasi, I R Brown
Mar 4, 2008·Molecular Cell·Peter D MarinerJames A Goodrich
Jan 1, 1985·CRC Critical Reviews in Biochemistry·E A Craig
Nov 8, 2015·Biochemistry. Biokhimii︠a︡·O L KantidzeS V Razin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.