PMID: 6166321May 26, 1981Paper

Regulated transport of messenger ribonucleic acid from isolated liver nuclei by nucleic acid binding proteins

Biochemistry
R B Moffett, T E Webb

Abstract

Rat liver nucleocytosolic messenger ribonucleic acid (mRNA) transport is shown to be regulated by proteins with a high affinity for nucleic acids. In the cell-free system described, the energy-dependent transport of all RNA classes [transfer RNA (tRNA), mRNA, and ribosomal RNA (rRNA)] exhibited a dependence upon the availability of discrete minor sets of cytosol proteins. In addition to having a different level of saturation, only the mRNA "transport protein" activities are increased by adenosine cyclic 3',5'-phosphate (cAMP), an effect most likely mediated by a cAMP-dependent protein kinase. The mRNA transport proteins were isolated from cytosol by precipitation with streptomycin sulfate followed by deoxyribonucleic acid (DNA)-cellulose affinity chromatography, or from oligo-(thymidylate)-cellulose bound cytoplasmic messenger ribonucleoprotein (mRNP) particles by high-salt extraction. Either method yielded a protein fraction which exhibited a 1000-fold increase in mRNA transport activity as compared to cytosol. Over one-half of the mRNA transport activity is associated with the mRNP of the cell. A partial homology between the cytosol and mRNP-derived proteins was demonstrated by polyacrylamide gel electrophoresis. One major (2...Continue Reading

References

Jul 1, 1977·European Journal of Biochemistry·A E SippelG Schütz
Feb 10, 1975·Biochimica Et Biophysica Acta·J D JohnsonJ Bonner
Sep 2, 1975·Biochemical and Biophysical Research Communications·T G ObrigJ J Ferguson
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Mar 1, 1977·Nucleic Acids Research·A YannarellT E Webb
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Citations

Jan 1, 1987·Molecular Biology Reports·C M Tsiapalis
Sep 30, 1985·Biochemical and Biophysical Research Communications·L Van HoveH Slegers
Aug 2, 1983·Biochimica Et Biophysica Acta·R B Moffett, T E Webb
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S Smart-NixonT E Webb
Aug 1, 1994·Trends in Cell Biology·P S Agutter
Jul 27, 1981·Biochimica Et Biophysica Acta·T PalayoorT E Webb
Mar 29, 1985·Biochemical and Biophysical Research Communications·M Hanausek-WalaszekT E Webb
Feb 1, 1987·Neurochemical Research·S TewariP Najarian
Mar 15, 1982·Biochemical and Biophysical Research Communications·D E Schumm, T E Webb

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