Regulating DSC2 Expression Affects the Proliferation and Apoptosis of Prostate Cancer Cells

Cancer Management and Research
Feng Jiang, Pengfei Wu

Abstract

Prostate cancer threatens the life and health of men in China. Desmocollin-2 (DSC2) is a member of DSC family, abnormal expression of which can affect the invasion and metastasis of tumor cells. The aim of this study was to investigate the role of DSC2 in prostate cancer. Regulating DSC2 expression in prostate cancer cells was conducted with transfection. The expression of DSC2, apoptosis-related proteins, cell cycle-related proteins and E-cadherin (E-cad)/β-catenin pathway was detected by Western blot analysis. The proliferation, clone formation ability, migration, invasion and apoptosis of transfected cells were in turn detected by cell counting kit-8 (CCK-8) assay, clone formation assay, wound healing assay, transwell assay and flow cytometry analysis. DSC2 expression was increased in prostate cancer cells compared with RWPE-1 cells. Inhibition of DSC2 promoted the proliferation, clone formation ability, migration and invasion while suppressed apoptosis of LNCaP cells and PC-3 cells. Inhibition of DSC2 affected the expression of apoptosis-related proteins and cell cycle-related proteins according to the changes of apoptosis and proliferation. Furthermore, inhibition of DSC2 up-regulated the expression of p-β-catenin and EGFR...Continue Reading

References

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Citations

Oct 12, 2021·Frontiers in Cell and Developmental Biology·Lisa MüllerRené Keil

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Methods Mentioned

BETA
transfection
PCR
flow cytometry
electrophoresis
Flow

Software Mentioned

SPSS
Quantity One
GEPIA

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