Regulation and Characterization of Mutants of fixABCX in Rhizobium leguminosarum.

Molecular Plant-microbe Interactions : MPMI
Isabel U C WebbP S Poole

Abstract

Symbiosis between Rhizobium leguminosarum and Pisum sativum requires tight control of redox balance in order to maintain respiration under the microaerobic conditions required for nitrogenase while still producing the eight electrons and sixteen molecules of ATP needed for nitrogen fixation. FixABCX, a cluster of electron transfer flavoproteins essential for nitrogen fixation, is encoded on the Sym plasmid (pRL10), immediately upstream of nifA, which encodes the general transcriptional regulator of nitrogen fixation. There is a symbiotically regulated NifA-dependent promoter upstream of fixA (PnifA1), as well as an additional basal constitutive promoter driving background expression of nifA (PnifA2). These were confirmed by 5'-end mapping of transcription start sites using differential RNA-seq. Complementation of polar fixAB and fixX mutants (Fix- strains) confirmed expression of nifA from PnifA1 in symbiosis. Electron microscopy combined with single-cell Raman microspectroscopy characterization of fixAB mutants revealed previously unknown heterogeneity in bacteroid morphology within a single nodule. Two morphotypes of mutant fixAB bacteroids were observed. One was larger than wild-type bacteroids and contained high levels of p...Continue Reading

References

Jul 22, 1975·Biochemical and Biophysical Research Communications·F H PettitL J Reed
Apr 1, 1975·Journal of General Microbiology·A W Johnston, J E Beringer
Jun 1, 1992·Journal of Bacteriology·P G AgronD R Helinski
Jul 1, 1987·Journal of Bacteriology·G DittaC H Kim
Dec 1, 1989·Microbiological Reviews·U Kües, U Stahl
Mar 1, 1988·Journal of Bacteriology·M Gubler, H Hennecke
Mar 1, 1987·Journal of Bacteriology·A M Hirsch, C A Smith
Sep 1, 1974·Journal of General Microbiology·J E Beringer
Aug 1, 1968·Journal of Bacteriology·I Friedberg, G Avigad
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
Dec 1, 1993·Journal of General Microbiology·G BodeP Malfertheiner
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·B BritoT Ruiz-Argüeso
May 11, 2002·Journal of Bacteriology·Turlough M Finan
Jun 14, 2002·FEBS Letters·Lionel Ferrières, Daniel Kahn
Mar 6, 2004·Molecular Plant-microbe Interactions : MPMI·Anke BeckerJacques Batut
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Sep 18, 2004·Journal of Bacteriology·Marta MartínezTomás Ruiz-Argüeso
Nov 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Melanie J BarnettSharon R Long
Jan 26, 2005·FEMS Microbiology Letters·Srinivas Reddy PallerlaSiegfried M Schoberth
Jan 28, 2005·Molecular Plant-microbe Interactions : MPMI·E M LodwigP S Poole
Mar 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Peter MergaertEva Kondorosi
Apr 4, 2007·FEMS Microbiology Letters·Traki BenhassineJan Michiels
Oct 24, 2007·Journal of Bacteriology·Andrea LindemannHans-Martin Fischer
May 11, 2010·Applied and Environmental Microbiology·Emmanuel SalazarSergio Encarnación
Oct 13, 2010·Biochimica Et Biophysica Acta·Nicholas J Watmough, Frank E Frerman
Jul 20, 2012·Molecular Plant-microbe Interactions : MPMI·David Zamorano-SánchezLourdes Girard
Mar 5, 2013·Annual Review of Plant Biology·Michael Udvardi, Philip S Poole

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.