Regulation and characterization of the galactose-phosphoenolpyruvate-dependent phosphotransferase system in Lactobacillus casei.

Journal of Bacteriology
B M Chassy, J Thompson

Abstract

Cells of Lactobacillus casei grown in media containing galactose or a metabolizable beta-galactoside (lactose, lactulose, or arabinosyl-beta-D-galactoside) were induced for a galactose-phosphoenolpyruvate-dependent phosphotransferase system (gal-PTS). This high-affinity system (Km for galactose, 11 microM) was inducible in eight strains examined, which were representative of all five subspecies of L. casei. The gal-PTS was also induced in strains defective in glucose- and lactose-phosphoenolpyruvate-dependent phosphotransferase systems during growth on galactose. Galactose 6-phosphate appeared to be the intracellular inducer of the gal-PTS. The gal-PTS was quite specific for D-galactose, and neither glucose, lactose, nor a variety of structural analogs of galactose caused significant inhibition of phosphotransferase system-mediated galactose transport in intact cells. The phosphoenolpyruvate-dependent phosphorylation of galactose in vitro required specific membrane and cytoplasmic components (including enzyme IIIgal), which were induced only by growth of the cells on galactose or beta-galactosides. Extracts prepared from such cells also contained an ATP-dependent galactokinase which converted galactose to galactose 1-phosphate....Continue Reading

References

Dec 14, 1976·Biochimica Et Biophysica Acta·P W Postma, S Roseman
Feb 1, 1972·Journal of Bacteriology·E R Kashket, T H Wilson
May 15, 1973·Biochemical and Biophysical Research Communications·D L Bissett, R L Anderson
Jun 10, 1968·Biochemical and Biophysical Research Communications·R D SimoniS Roseman
Jun 1, 1968·Journal of Bacteriology·M L MorseW Hengstenberg
Aug 1, 1981·Journal of Bacteriology·J Thompson, B M Chassy
Nov 1, 1980·Journal of Bacteriology·J Thompson
May 1, 1962·The Journal of Infectious Diseases·C EFTHYMIOU, P A HANSEN
Aug 1, 1978·Applied and Environmental Microbiology·L L McKay, K A Baldwin
Jan 1, 1978·Current Microbiology·B M ChassyA Guiffrida

❮ Previous
Next ❯

Citations

Jan 1, 1990·Critical Reviews in Food Science and Nutrition·P F FoxT M Cogan
May 28, 2013·Applied and Environmental Microbiology·Pablo MorteraJuke S Lolkema
Oct 1, 1994·FEMS Microbiology Reviews·W M de Vos, E E Vaughan
May 7, 2004·European Journal of Biochemistry·Manuela PailChristian P Kubicek
Jun 5, 2012·Applied and Environmental Microbiology·Lin ZengRobert A Burne
May 1, 2013·Critical Reviews in Food Science and Nutrition·Paulraj KanmaniVenkatesan Arul
Dec 30, 1999·Journal of Bacteriology·M J YebraG Pérez-Martínez
Jun 3, 1998·Applied and Environmental Microbiology·K Bettenbrock, C A Alpert
Dec 1, 1987·Applied and Environmental Microbiology·M Shimizu-Kadota
Oct 1, 1984·Journal of Bacteriology·S Z HausmanJ London
Sep 1, 1985·Microbiological Reviews·P W Postma, J W Lengeler
Apr 1, 1986·Applied and Environmental Microbiology·M W HickeyG R Jago
Sep 1, 1993·Microbiological Reviews·P W PostmaG R Jacobson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.